Plasminogen activation in epiretinal membranes
| Autor: | Ilkka Immonen, Antti Vaheri, Petri Tommila, Vappu Sirén |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Proliferative vitreoretinopathy Plasmin Biology Extracellular matrix Cicatrix Plasminogen Activators 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Humans Zymography Fibrinolysin Fluorescent Antibody Technique Indirect Aged 030304 developmental biology Aged 80 and over Urokinase 0303 health sciences Membranes Vitreoretinopathy Proliferative Retinal Detachment Plasminogen Anatomy Middle Aged medicine.disease Sensory Systems Extracellular Matrix Cell biology Vitreous Body Ophthalmology Membrane 030221 ophthalmology & optometry Female Epiretinal membrane Plasminogen activator Cell Division medicine.drug |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 234:664-669 |
| ISSN: | 1435-702X 0721-832X |
| DOI: | 10.1007/bf00292351 |
| Popis: | • Background: Formation of epiretinal membranes occurs in proliferative vitreoretinopathy, macular pucker and after penetrating trauma. Epiretinal membrane formation includes cell migration and proliferation, extracellular matrix formation and tissue contraction. Generally in scar tissue formation, the production of new extracellular matrix occurs concomitantly with its proteolytic degradation, resulting in continuous tissue remodelling. The plasminogen activator-mediated proteolytic cascade is an important mechanism for pericellular degradation of the extracellular matrix. Therefore we wanted to study the presence of the plasminogen activator-mediated proteolytic cascade in epiretinal membranes. • Methods: Specimens of 18 epiretinal and 3 subretinal membranes were obtained during vitreous surgery for retinal detachment with proliferative vitreoretinopathy or macular pucker. Plasminogen activators and plasmin were characterized in frozen sections of epiretinal membranes by in situ zymography and in membrane lysates by zymography. Indirect immunofluorescence staining was performed to localize urokinase in epiretinal membranes. • Results: Urokinase was present in 17/21 and tissue-type plasminogen activator in 12/21 of the membranes studied. Active plasmin was not detected in the frozen sections of epiretinal membranes. Immunofluorescence staining localized urokinase predominantly in the areas invaded by macrophages and cells of retinal pigment epithelial origin. • Conclusion: Our results demonstrate the presence of proteolytic activity in periretinal scar tissue. Urokinase was more consistently present, but smaller amounts of tissue-type plasminogen activator were also found in the specimens. These results indicate that continuous tissue remodelling with simultaneous extracellular matrix production and breakdown regulates the growth of epiretinal membranes. |
| Databáze: | OpenAIRE |
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