The amino acids of Autographa californica multiple nucleopolyhedrovirus P48 critical for the association with Ac93 are important for the nuclear egress of nucleocapsids and efficient formation of intranuclear microvesicles
| Autor: | Wenbi Wu, Junjie He, Yan Wang, Kai Yang, Meijin Yuan, Mei Mo, Qingyun Cai |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
viruses Morphogenesis Biology Spodoptera medicine.disease_cause Virus Replication Virology medicine Animals Amino Acids Nucleocapsid Gene Alanine chemistry.chemical_classification Cell Nucleus Mutation Microvesicle fungi biochemical phenomena metabolism and nutrition biology.organism_classification Microvesicles Nucleopolyhedroviruses Cell biology Amino acid Autographa californica Infectious Diseases chemistry |
| Zdroj: | Virus research. 308 |
| ISSN: | 1872-7492 |
| Popis: | Autographa californica multiple nucleopolyhedrovirus (AcMNPV) undergoes a biphasic life cycle with the production of two physically and functionally distinct virions: budded virions (BVs) and occlusion-derived virions (ODVs). Nuclear egress of nucleocapsids and intranuclear microvesicle formation are critical for the morphogenesis of BVs and ODVs, respectively, but the mechanisms and details of these two processes remain unknown. Our previous studies have shown that AcMNPV p48 (ac103) gene is essential for the nuclear egress of nucleocapsids and efficient formation of intranuclear microvesicles, and protein P48 associates with Ac93, which is also involved in the above processes in virion morphogenesis. In this study, we present evidence that alanine substitution for residues N318, V319, C320, R321, and I323 of P48 disrupted the association with Ac93. Moreover, mutation of these residues blocked the nuclear egress of nucleocapsids and efficient formation of intranuclear microvesicles, and subsequent BV formation, as well as ODV envelopment and embedding of ODVs into polyhedra. These results suggested that the association between P48 and Ac93 may be important for both BV and ODV morphogenesis. |
| Databáze: | OpenAIRE |
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