Immunohistochemical expression of programmed death-ligand 1 and CD8 in glioblastomas
| Autor: | Khaled Abdel Karim Mohamed, Zeinab A. Kamar, Dina Mohamed El Samman, Manal M. El Mahdy, Hoda H Abou Gabal, Hala Sobhy Cousha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Histology
Tumor-infiltrating lymphocytes business.industry glioblastoma cd8 Ligand (biochemistry) Pathology and Forensic Medicine Blockade survival analysis Immune system programmed death ligand 1 tumor-infiltrating lymphocytes Cancer research Pathology Immunohistochemistry Medicine RB1-214 Original Article Receptor business CD8 Survival analysis |
| Zdroj: | Journal of Pathology and Translational Medicine, Vol 55, Iss 6, Pp 388-397 (2021) Journal of Pathology and Translational Medicine |
| ISSN: | 2383-7845 2383-7837 |
| Popis: | Background Glioblastoma is the most aggressive primary malignant brain tumor in adults and is characterized by poor prognosis. Immune evasion occurs via programmed death-ligand 1 (PD-L1)/programmed death receptor 1 (PD-1) interaction. Some malignant tumors have responded to PD-L1/PD-1 blockade treatment strategies, and PD-L1 has been described as a potential predictive biomarker. This study discussed the expression of PD-L1 and CD8 in glioblastomas. Materials and methods Thirty cases of glioblastoma were stained immunohistochemically for PD-L1 and CD8, where PD-L1 expression in glioblastoma tumor tissue above 1% is considered positive and CD-8 is expressed in tumor infiltrating lymphocytes. The expression of each marker was correlated with clinicopathologic parameters. Survival analysis was conducted to correlate progression-free survival (PFS) and overall survival (OS) with PD-L1 and CD8 expression. Results Diffuse/fibrillary PD-L1 was expressed in all cases (mean expression, 57.6%), whereas membranous PD-L1 was expressed in six of 30 cases. CD8-positive tumor-infiltrating lymphocytes (CD8+ TILs) had a median expression of 10%. PD-L1 and CD8 were positively correlated (p = .001). High PD-L1 expression was associated with worse PFS and OS (p = .026 and p = .001, respectively). Correlation of CD8+ TILs percentage with age, sex, tumor site, laterality, and outcomes were statistically insignificant. Multivariate analysis revealed that PD-L1 was the only independent factor that affected prognosis. Conclusion PD-L1 expression in patients with glioblastoma is robust; higher PD-L1 expression is associated with lower CD8+ TIL expression and worse prognosis. |
| Databáze: | OpenAIRE |
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