Establishment of monoclonal antibody NI-58 which inhibits homotypic cell adhesion of LPS-stimulated U937 cells
| Autor: | Hidekazu Tamauchi, Hidetoshi Inoko, Takeshi Sasahara, Nobunao Ikewaki |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Monocytes CD49b Mice Antigen hemic and lymphatic diseases Cell Adhesion Animals Cell adhesion Antigen-presenting cell Cells Cultured CD40 biology Chemistry Cell adhesion molecule Antibodies Monoclonal General Medicine Molecular biology Cell biology Molecular Weight Depression Chemical Immunoglobulin G Antigens Surface biology.protein Neural cell adhesion molecule Antibody |
| Zdroj: | Nippon Saikingaku Zasshi. 48:429-433 |
| ISSN: | 1882-4110 0021-4930 |
| DOI: | 10.3412/jsb.48.429 |
| Popis: | A mouse monoclonal IgG1 antibody, referred to as NI-58, has been produced. In immunofluorescence assay, this antibody reacted with myelomonocytes, EBV-B cells, Burkitt's lymphoma cells, T cell leukemia cells and peripheral blood mononuclear cells, but not with erythroid cells. The surface antigen on U937 cells recognized by NI-58 had a molecular size of 65 kDa as determined by immunoblotting analysis. As a biological function, NI-58 strongly inhibited the homotypic cell adhesion of LPS-stimulated U937 cells. It was found that the antigen defined by NI-58 was distinct from CD54 (intercellular adhesion molecule-1) in it's pattern of cellular expression and molecular weight, suggesting that NI-58 recognizes a new adhesion molecule and inhibits the homotypic cell adhesion of LPS-stimulated U937 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|