Lipid domain–dependent regulation of single-cell wound repair
| Autor: | Troy A. Hornberger, Emily M. Vaughan, Nicolas Vitale, William M. Bement, Jae-Sung You, Hoi-Ying Elsie Yu, Amber Lasek |
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| Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), R01 GM052932 |
| Rok vydání: | 2014 |
| Předmět: |
Diacylglycerol Kinase
Transferases (Other Substituted Phosphate Groups) macromolecular substances CDC42 GTPase Xenopus Proteins Biology Cell Membrane Structures Diglycerides Xenopus laevis 03 medical and health sciences 0302 clinical medicine Single-cell analysis Phospholipase D medicine Animals Molecular Biology Cell damage Cytoskeleton Protein kinase C Monomeric GTP-Binding Proteins 030304 developmental biology Diacylglycerol kinase 0303 health sciences Articles Cell Biology medicine.disease 3. Good health Transport protein Cell biology Protein Transport Type C Phospholipases Oocytes Phosphatidylcholines [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] lipids (amino acids peptides and proteins) Single-Cell Analysis Rho Guanine Nucleotide Exchange Factors 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2014, 25 (12), pp.1867-1876. ⟨10.1091/mbc.E14-03-0839⟩ |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e14-03-0839 |
| Popis: | Cell repair is a conserved and medically important process. Cell damage triggers the rapid accumulation of several different lipids around wounds, and the lipids sort into distinct domains around them. One of these lipids—diacylglycerol—is required for activation of Rho and Cdc42 and healing. After damage, cells reseal their plasma membrane and repair the underlying cortical cytoskeleton. Although many different proteins have been implicated in cell repair, the potential role of specific lipids has not been explored. Here we report that cell damage elicits rapid formation of spatially organized lipid domains around the damage site, with different lipids concentrated in different domains as a result of both de novo synthesis and transport. One of these lipids—diacylglycerol (DAG)—rapidly accumulates in a broad domain that overlaps the zones of active Rho and Cdc42, GTPases that regulate repair of the cortical cytoskeleton. Formation of the DAG domain is required for Cdc42 and Rho activation and healing. Two DAG targets, protein kinase C (PKC) β and η, are recruited to cell wounds and play mutually antagonistic roles in the healing process: PKCβ participates in Rho and Cdc42 activation, whereas PKCη inhibits Rho and Cdc42 activation. The results reveal an unexpected diversity in subcellular lipid domains and the importance of such domains for a basic cellular process. |
| Databáze: | OpenAIRE |
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