The identification of monoclonal class switch variants by sib selection and an ELISA assay
| Autor: | Antonio Bargellesi, Jean Luc Teillaud, Matthew D. Scharff, Gad Spira |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Surface Immunoglobulin
medicine.drug_class Immunology Immunoglobulin Variable Region Enzyme-Linked Immunosorbent Assay Computational biology Monoclonal antibody Subclass Cell Line Mice Antigen medicine Animals Immunology and Allergy Genetics Mice Inbred BALB C Hybridomas medicine.diagnostic_test biology Antibodies Monoclonal Isotype Immunoglobulin M Immunoglobulin G Immunoassay Monoclonal biology.protein Immunoglobulin Light Chains Antibody Immunoglobulin Heavy Chains Plasmacytoma |
| Zdroj: | Journal of Immunological Methods. 74:307-315 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(84)90298-9 |
| Popis: | Monoclonal antibodies can now be generated against a wide variety of antigens. However, a potentially useful monoclonal antibody may be of the wrong class or subclass for a particular task. Antibodies of the desired class can be obtained by identifying rare subclones in which the variable region has been rearranged to a new constant region. We describe here the use of sib selection and an enzyme-linked immunoassay (ELISA) for isolating such class and subclass switch variants from 2 IgM and 1 IgG 3 producing hybridoma. The relative simplicity of ELISA assays make it feasible to apply this approach to many different hybridomas. Since commercial affinity purified class and subclass specific antibodies are now available, the method can be used by any laboratory. Furthermore, the technique does not require that the switch variants express surface immunoglobulin and enriches for hybridomas secreting higher amounts of antibody. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|