Determination of levodopa in human plasma by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS): application to a bioequivalence study
| Autor: | Douglas Pereira Pinto, C. Amendoeira, A. S. Marques, Heliana Figueiredo Martins, A. Nascimento |
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| Rok vydání: | 2013 |
| Předmět: |
bioequivalence
Chromatography Formic acid Analytical chemistry General Chemistry Bioequivalence Tandem mass spectrometry High-performance liquid chromatography lcsh:Chemistry Bioequivalence study chemistry.chemical_compound lcsh:QD1-999 chemistry Human plasma HPLC-ESI-MS/MS Perchloric acid Methanol levodopa |
| Zdroj: | Química Nova, Vol 36, Iss 1, Pp 171-176 (2013) |
| ISSN: | 0100-4042 |
| DOI: | 10.1590/s0100-40422013000100028 |
| Popis: | A sensitive, accurate and simple method using HPLC-MS/MS was developed and validated for levodopa quantitation in human plasma. Analysis was achieved on a pursuit® C18 analytical column (5 µm; 150 x 4.6 mm i.d.) using a mobile phase (methanol and water , 90:10, v/v) containing formic acid 0.5% v/v, after extracting the samples using a simple protein plasma precipitation with perchloric acid. The developed method was validated in accordance with ANVISA guidelines and was successfully applied to a bioequivalence study in 60 healthy volunteers demonstrating the feasibility and reliability of the proposed method. |
| Databáze: | OpenAIRE |
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