Epi-ID: Systematic and Direct Screening for Chromatin Regulators in Yeast by Barcode-ChIP-Seq
| Autor: | Deepani W. Poramba-Liyanage, Tessy Korthout, Fred van Leeuwen |
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| Přispěvatelé: | Graduate School, ACS - Heart failure & arrhythmias |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493997350 Methods in Molecular Biology, 2049, 87-103 |
| Popis: | The assembly and regulation of chromatin requires coordinated activity of multiple mechanisms. Many factors feed into signaling networks that control the epigenome of a cell. It is this complexity that makes understanding the layers of epigenetic regulation a challenge. Genetic screens have been indispensable for studying chromatin processes. However, they can be laborious and the readout for chromatin changes is often indirect. Epi-ID is a screening strategy in yeast that enables the direct assessment of chromatin status in thousands of gene mutants in parallel. Epi-ID takes advantage of DNA sequences called DNA barcodes that are introduced into a library of yeast knockout mutants at a common chromosomal location in the genome. Chromatin immunoprecipitation on pools of barcoded mutant strains followed by barcode counting by high throughput sequencing will report on the abundance of the chromatin mark of interest in each mutant strain. Epi-ID is applicable to a wide range of chromatin proteins and modifications that are present and can be immunoprecipitated at or around the barcoded region. |
| Databáze: | OpenAIRE |
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