Rapid microwave-based method for the preparation of antimicrobial lignin-capped silver nanoparticles active against multidrug-resistant bacteria
| Autor: | Horacio Bach, Yael N. Slavin, Jason Asnis, Katayoun Saatchi, Robert E. W. Hancock, Urs O. Häfeli, Daniel Pletzer |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Silver medicine.drug_class Antibiotics Metal Nanoparticles Pharmaceutical Science Microbial Sensitivity Tests 02 engineering and technology medicine.disease_cause Lignin 030226 pharmacology & pharmacy Silver nanoparticle Microbiology Mice 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine Anti-Infective Agents In vivo medicine Animals Microwaves biology Chemistry Pseudomonas aeruginosa Pseudomonas 021001 nanoscience & nanotechnology Antimicrobial biology.organism_classification Anti-Bacterial Agents Staphylococcus aureus 0210 nano-technology |
| Zdroj: | International Journal of Pharmaceutics. 596:120299 |
| ISSN: | 0378-5173 |
| Popis: | Due to the increasing inability of antibiotics to treat multidrug-resistant (MDR) bacteria, metal and metal oxide nanoparticles have been gaining interest as antimicrobial agents. Among those, silver nanoparticles have been used extensively as broad-spectrum antimicrobial agents. Here, we describe a newly-developed, 10-min (120 °C at 5 bar pressure) microwave-assisted synthesis of silver nanoparticles made from the wood biopolymer lignin as a reducing and capping agent. The resulting lignin-capped silver nanoparticles (AgLNPs) had an average particle diameter of 13.4 ± 2.8 nm. Antimicrobial susceptibility assays against a variety of MDR clinical Gram-positive and Gram-negative pathogens revealed a minimal inhibitory concentration (MIC) of AgLNPs ≤5 µg/mL. AgLNPs (10 µg/mL) showed ≤20% cytotoxicity towards monocytic THP-1 cells and were well tolerated when administered subcutaneously in mice at high concentrations (5 mg at a concentration of 100 mg/mL) with no obvious toxicity. AgLNPs showed efficacy in an in vivo infection (abscess) mouse model against MDR Pseudomonas aeruginosa LESB58 and methicillin-resistant Staphylococcus aureus USA300. A significant decrease in abscess sizes was observed for both strains as well as a reduction in bacterial loads of P. aeruginosa after three days. This demonstrates that microwave-assisted synthesis provides an optimized strategy for the production of AgLNPs while maintaining antimicrobial activity in vitro and in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect