Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion
| Autor: | Peishan Hu, Jiangang Yuan, Xiaozhong Peng, Xihua Lin, Bin Yang, Zhaoshi Bao, Wei Liu, Fan Wu, Tao Jiang, Wei Han, Boqin Qiang, Xiaochao Tan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Male Transplantation Heterologous Regulator Mice Nude RNA-binding protein Plasma protein binding ARHGDIA 03 medical and health sciences Young Adult RNA interference Cell Line Tumor glioma microRNA Medicine Animals Humans Metastasis suppressor RNA Messenger PCBP2 3' Untranslated Regions Aged miRNA rho Guanine Nucleotide Dissociation Inhibitor alpha Messenger RNA Mice Inbred BALB C business.industry Three prime untranslated region Brain Neoplasms RNA-Binding Proteins Middle Aged migration and invasion Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Oncology Immunology Cancer research Female RNA Interference business HeLa Cells Protein Binding Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | RNA-RNA and protein-RNA interactions are essential for post-transcriptional regulation in normal development and may be deregulated in cancer initiation and progression. The RNA-binding protein PCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, stability and activity. Here, we identified Rho GDP dissociation inhibitor α (ARHGDIA) as a target mRNA that binds to PCBP2, and we uncovered the role of ARHGDIA as a putative metastasis suppressor through analyses of in vitro and in vivo models of EMT and metastasis. Furthermore, we demonstrated that ARHGDIA is a potential target of miR-151-5p and miR-16 in gliomas. The interaction between PCBP2 and the 3'UTR of the ARHGDIA mRNA may induce a local change in RNA structure that favors subsequent binding of miR-151-5p and miR-16, thus leading to the suppression of ARHGDIA expression. PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA. |
| Databáze: | OpenAIRE |
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