Combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR to Monitor In Vitro Vitetrifolin D Phase I and II Metabolism
| Autor: | Hermann Stuppner, Christina Högner, Sonja Sturm, Christoph Seger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Endocrinology
Diabetes and Metabolism HR-mass spectrometry vitetrifolin D Microbiology Biochemistry Vitex agnus-castus High-performance liquid chromatography Article Phase (matter) Molecular Biology Chromatography biology Chemistry In vitro metabolism Liver cell structure elucidation Metabolism biology.organism_classification QR1-502 In vitro HPLC-DAD-QTOF-MS metabolism Hplc dad liver microsomes HPLC-SPE-NMR |
| Zdroj: | Metabolites Volume 11 Issue 8 Metabolites, Vol 11, Iss 529, p 529 (2021) |
| ISSN: | 2218-1989 |
| DOI: | 10.3390/metabo11080529 |
| Popis: | By combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR, the in vitro metabolism of vitetrifolin D, a pharmacologically active key molecule from Vitex agnus-castus in liver cell fractions, was investigated. Twenty-seven phase I and phase II metabolites were tentatively identified from the culture broth by HPLC-DAD-QTOF-MS. The subsequent HPLC-SPE-NMR analysis allowed for the unequivocal structural characterization of nine phase I metabolites. Since the preparative isolation of the metabolites was avoided, the substance input was much lower than in conventional strategies. The study did prove that the use of hyphenated instrumental analysis methodologies allows for the successful performance of in vitro metabolism studies, even if the availability of substances is very limited. |
| Databáze: | OpenAIRE |
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