Increased HLA-DR Expression on Tissue Eosinophils in Eosinophilic Esophagitis
| Autor: | Anup Patel, Yael Gernez, Kari C. Nadeau, John A. Kerner, William E. Berquist, Eric Sibley, Tammie Nguyen, Kenneth L. Cox, Dorsey Bass, Judy Fuentebella |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Young Adult Esophagus medicine HLA-DR Humans Child Eosinophilic esophagitis Esophageal disease business.industry Gastroenterology Infant Eosinophilic Esophagitis HLA-DR Antigens Eosinophil medicine.disease Eosinophils medicine.anatomical_structure Child Preschool Pediatrics Perinatology and Child Health Gastroesophageal Reflux GERD Immunohistochemistry Female business Esophagitis |
| Zdroj: | Journal of Pediatric Gastroenterology & Nutrition. 51:290-294 |
| ISSN: | 0277-2116 |
| DOI: | 10.1097/mpg.0b013e3181e083e7 |
| Popis: | OBJECTIVE The aim of the study was to investigate whether eosinophils have increased human leukocyte antigen (HLA)-DR expression in subjects with eosinophilic esophagitis (EoE) compared with controls. PATIENTS AND METHODS Patients who were undergoing an upper endoscopy with biopsies for suspected gastroesophageal reflux disease (GERD) or EoE at Lucile Packard Children's Hospital were enrolled. In total, the blood and tissue samples of 10 healthy controls (HC), 11 subjects with GERD, and 10 with EoE were studied. Multiple tissue staining to identify eosinophils (via eosinophil cationic protein-clone EG2) and major histocompatibility complex class II cell surface receptors (via HLA-DR) was performed via immunohistochemistry. The peripheral blood was analyzed using flow cytometry to detect eosinophil HLA-DR expression among these subjects. RESULTS In the tissue, a greater proportion of eosinophils expressed HLA-DR among the subjects with EoE (mean 0.83 +/- 0.14, n = 9) relative to those with GERD (mean 0.18 +/- 0.19, n = 8, P < 0.01) and HC (mean 0.18 +/- 0.13, n = 6, P < 0.01). In total, 6 participants (4 HC subjects and 2 subjects with GERD) did not have any eosinophils identified on tissue staining and were unable to be included in the present statistical analysis. In the blood, there was no statistically significant difference in eosinophil HLA-DR expression among HC subjects (mean 415 +/- 217, n = 6), subjects with GERD (mean 507 +/- 429, n = 2), and those with EoE (mean 334 +/- 181, n = 6). CONCLUSIONS These data demonstrate that the eosinophils from the esophagus of subjects with EoE have increased HLA-DR expression within this tissue. |
| Databáze: | OpenAIRE |
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