Analysis of Tg transcripts by real-time RT-PCR in the blood of thyroid cancer patients
| Autor: | J. C. Bigorgne, Frédérique Savagner, Yves Malthièry, Vincent Rohmer, Pascal Reynier, Patrice Rodien |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Goiter Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biochemistry Thyroglobulin Endocrinology Computer Systems Reference Values Internal medicine Adenocarcinoma Follicular medicine Humans Postoperative Period RNA Messenger Thyroid Neoplasms Thyroid cancer Immunoradiometric assay biology business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) Thyroid Thyroidectomy medicine.disease Carcinoma Papillary Alternative Splicing medicine.anatomical_structure Real-time polymerase chain reaction biology.protein Adenocarcinoma Female Immunoradiometric Assay Antibody business Follow-Up Studies Goiter Nodular |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 87(2) |
| ISSN: | 0021-972X |
| Popis: | Serum Tg (sTg) assays are sometimes unsatisfactory for monitoring thyroid cancer because interference caused by anti-Tg antibodies may reduce the sensitivity of the tests during thyroid hormone therapy. We have therefore developed a complementary method using real-time quantitative RT-PCR based on the amplification of Tg mRNA. Two different pairs of primers were used for the determination of the frequency of one of the variants of the alternative splicing of Tg mRNA. The frequency of this variant was as high in patients (n = 40) as in controls (n = 30), accounting for about 33% of the total Tg mRNA. Using appropriate primers, we observed that Tg mRNA values in controls varied according to the volume of thyroid tissue and the TSH concentration. The Tg mRNA values allowed the definition of a positive cutoff point at 1 pg/microg total RNA. This cutoff point, tested on the group of patients treated for thyroid cancer, produced fewer false negative results than those obtained with sTg assays. The standardized, highly sensitive real-time RT-PCR technique may therefore prove useful as a complement to sTg assays, particularly for patients with recurrent thyroid cancer receiving T(4) therapy. |
| Databáze: | OpenAIRE |
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