Performance characteristics of an ELISA screening assay for urinary synthetic cannabinoids
| Autor: | Allan J. Barnes, Thomas M. Martin, Marilyn A. Huestis, Marisol S. Castaneto, Kevin L. Klette, Sheena Young, Eliani Spinelli |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.drug_class
medicine.medical_treatment Urinary system Pharmaceutical Science Enzyme-Linked Immunosorbent Assay Urine Sensitivity and Specificity Analytical Chemistry Designer Drugs Limit of Detection Tandem Mass Spectrometry Synthetic cannabinoids medicine Environmental Chemistry Humans Spectroscopy Detection limit Chromatography medicine.diagnostic_test Chemistry Cannabinoids Screening assay Designer drug Substance Abuse Detection Immunoassay Cannabinoid medicine.drug Chromatography Liquid |
| Zdroj: | Drug testing and analysis. 7(6) |
| ISSN: | 1942-7611 |
| Popis: | Synthetic cannabinoids are marketed as legal alternatives to cannabis, as routine urine cannabinoid immunoassays do not detect synthetic cannabinoids. Laboratories are challenged to identify these new designer drugs that are widely available and represent a major public health and safety problem. Immunoassay testing offers rapid separation of presumptive positive and negative specimens, prior to more costly and time-consuming chromatographic confirmation. The Neogen SPICE ELISA kit targets JWH-018N-pentanoic acid as a marker for urinary synthetic cannabinoids. Assay performance was evaluated by analyzing 2469 authentic urine samples with the Neogen immunoassay and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two immunoassay cut-off concentrations, 5 and 10 μg/L, classified samples as presumptive positive or negative, followed by qualitative LC-MS/MS confirmation for 29 synthetic cannabinoids markers with limits of detection of 0.5–10 μg/L to determine the assay’s sensitivity, specificity and efficacy. Challenges at ±25% of each cut-off also were investigated to determine performance around the cut-off and intra- and inter-plate imprecision. The immunoassay was linear from 1 to 250 μg/L (r 2 =0.992) with intra- and inter-plate imprecision of ≤5.3% and |
| Databáze: | OpenAIRE |
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