Antimicrobial effects of root canal medicaments againstEnterococcus faecalisandStreptococcus mutans

Autor: Pinar Yurdakul, Dolunay Gülmez, B. Atila-Pektaş, Ömer Görduysus
Rok vydání: 2012
Předmět:
Zdroj: International Endodontic Journal. 46:413-418
ISSN: 0143-2885
DOI: 10.1111/iej.12004
Popis: Aim To compare the antimicrobial activities of Activ Point (Roeko, Langenau, Germany), Calcium Hydroxide Plus Point (Roeko, Langenau, Germany), calcium hydroxide, 1% chlorhexidine gel and bioactive glass (S53P4) against Enterococcus faecalis and Streptococcus mutans. Methodology One hundred and twenty extracted single-rooted human teeth were used. After removing the crowns, root canals were prepared by using the Protaper rotary system. Following autoclave sterilization, root canals were incubated at 37 °C with E. faecalis ATCC 29212 and S. mutans RSHM 676 for 1 week. The specimens, which were divided into five treatment groups for each microorganism according to the intracanal medicament used, were tested in 10 experimental runs. In each experimental run, 10 roots were included as treatment, one root as positive control and one root as sterility control. Sterile paper points were utilized to take samples from root canals after the incubation of teeth in thioglycollate medium at 37 °C for 1 week. Samples taken from teeth by sterile paper points were inoculated onto sheep blood agar, and following an overnight incubation, the colonies grown on sheep blood agar were counted and interpreted as colony-forming units. Results were tested statistically by using Kruskal–Wallis and Conover's nonparametric multiple comparison tests. Results CHX gel (P
Databáze: OpenAIRE