Lps induced cytokine production and expression of LPS-receptors by peripheral blood mononuclear cells of patients with familial hypercholesterolemia and the effect of HMG-CoA reductase inhibitors
| Autor: | N. de Bont, Mihai G. Netea, Tineke J. Smilde, C.P. Rovers, J.W.M. van der Meer, Pierre N.M. Demacker, A.F.H. Stalenhoef |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Heterozygote Simvastatin CD14 medicine.medical_treatment The effect of modulation of endogenous cytokines on resistance to infection Lipopolysaccharide Receptors Familial hypercholesterolemia Biology Cholesterolmetabolisme en cytokinen Peripheral blood mononuclear cell Monocytes Proinflammatory cytokine Cohort Studies Hyperlipoproteinemia Type II Mice Double-Blind Method Internal medicine medicine Atorvastatin Animals Pyrroles Receptor Cholesterol metabolism and cytokines CD11 Antigens Anticholesteremic Agents medicine.disease Lipids Het effect van modulatie van endogene cytokinen op weerstand tegen infecties Cytokine Endocrinology Heptanoic Acids Immunology HMG-CoA reductase biology.protein Cytokines Tumor necrosis factor alpha Hydroxymethylglutaryl-CoA Reductase Inhibitors Cardiology and Cardiovascular Medicine |
| Zdroj: | Atherosclerosis, 139, 147-152 Atherosclerosis, 139, 147-152. Elsevier sci ireland ltd Atherosclerosis, 139, pp. 147-152 Atherosclerosis, 139, 1, pp. 147-152 |
| ISSN: | 0021-9150 |
| Popis: | Inflammatory processes play an important role in atherogenesis, and proinflammatory cytokines such as interleukin-1beta (IL-1beta), IL-6, and tumour necrosis factor alpha (TNFalpha) are thought to be mediators in this phenomenon. We have previously established that peritoneal macrophages of LDL-receptor knock-out mice, which are hypercholesterolemic and are prone to atherosclerosis, have an increased LPS-induced cytokine production capacity, ex vivo. The aim of the present study was to investigate whether the process leading to atherosclerosis in patients with familial hypercholesterolemia (FH) is associated with increased cytokine production capacity of peripheral blood mononuclear cells (PBMC) and/or increased expression of adhesion molecules on monocytes and lymphocytes. Furthermore, we assessed the effect of cholesterol lowering on the production capacity of PBMC, as these drugs are beneficial with regard to cardiovascular diseases. LPS-induced IL-1beta and TNFalpha production by PBMCs of 21 heterozygous FH patients appeared to be similar to the production by PBMCs of 21 healthy volunteers. In addition, expression of the LPS-receptors CD14 and beta2-integrins in nine patients and controls did not differ either. In a second series of experiments, HMG-CoA synthesis inhibitors were ineffective to change the LPS-induced production by PBMC of IL-1alpha, IL-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, and TNFalpha. In conclusion, cytokine production capacity of blood cells or the expression of LPS-receptors on circulating PBMC do not deviate in subjects with FH and also do not change as a result of treatment with cholesterol synthesis inhibitors. |
| Databáze: | OpenAIRE |
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