Streptococcus pneumoniae Cell Wall-Localized Trigger Factor Elicits a Protective Immune Response and Contributes to Bacterial Adhesion to the Host
| Autor: | Shani Troib, Ronald J. Ellis, Vered Chalifa-Caspi, Hastyar Najmuldeen, Rachel Benisty, Hasan Yesilkaya, Shahar Dotan, Marilou Shagan, Michael Tal, Hannie Nachmani, Ron Dagan, Yaffa Mizrachi Nebenzahl, Maxim Portnoi, Aviad Cohen, Tatyana Kushnir |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Immunoblotting lcsh:Medicine Virulence medicine.disease_cause Bacterial Adhesion Article Microbiology law.invention Pathogenesis 03 medical and health sciences Mice 0302 clinical medicine Immune system law Cell Wall Streptococcus pneumoniae medicine Animals lcsh:Science Antiserum Mice Inbred BALB C Multidisciplinary biology lcsh:R Computational Biology Peptidylprolyl Isomerase Flow Cytometry In vitro 3. Good health 030104 developmental biology Chaperone (protein) Recombinant DNA biology.protein lcsh:Q Female 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Trigger factor (TF) has a known cytoplasmic function as a chaperone. In a previous study we showed that pneumococcal TF is also cell-wall localized and this finding combined with the immunogenic characteristic of TF, has led us to determine the vaccine potential of TF and decipher its involvement in pneumococcal pathogenesis. Bioinformatic analysis revealed that TF is conserved among pneumococci and has no human homologue. Immunization of mice with recombinant (r)TF elicited a protective immune response against a pneumococcal challenge, suggesting that TF contributes to pneumococcal pathogenesis. Indeed, rTF and an anti-rTF antiserum inhibited bacterial adhesion to human lung derived epithelial cells, indicating that TF contributes to the bacterial adhesion to the host. Moreover, bacteria lacking TF demonstrated reduced adhesion, in vitro, to lung-derived epithelial cells, neural cells and glial cells. The reduced adhesion could be restored by chromosomal complementation. Furthermore, bacteria lacking TF demonstrated significantly reduced virulence in a mouse model. Taken together, the ability of rTF to elicit a protective immune response, involvement of TF in bacterial adhesion, conservation of the protein among pneumococcal strains and the lack of human homologue, all suggest that rTF can be considered as a future candidate vaccine with a much broader coverage as compared to the currently available pneumococcal vaccines. |
| Databáze: | OpenAIRE |
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