Neutrophil lipoxygenase activation and leukosequestration in postischemic myocutaneous flaps: role of LTB4
| Autor: | John Mihran Davis, J. J. C. Chiao, B. S. Fyfe, Lloyd A. Hoffman, Gary A. Fantini, R. E. Kirschner |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Neutrophils Swine Physiology Leukotriene B4 Neutrophile Receptors Leukotriene B4 Ischemia chemistry.chemical_element Blood Pressure Calcium Surgical Flaps Leukocyte Count chemistry.chemical_compound Lipoxygenase Heart Rate Physiology (medical) Internal medicine medicine Animals Benzopyrans Lipoxygenase Inhibitors Muscle Skeletal Skin Arachidonate 5-Lipoxygenase biology Antagonist hemic and immune systems medicine.disease Enzyme Activation Endocrinology chemistry Eicosanoid Reperfusion Immunology Arachidonate 5-lipoxygenase biology.protein Female Cardiology and Cardiovascular Medicine |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 268:H2167-H2174 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1995.268.6.h2167 |
| Popis: | Reperfusion of ischemic tissues leads to eicosanoid- and polymorphonuclear leukocyte (PMN)-dependent injury. The present experiments were undertaken to examine the effect of myocutaneous flap ischemia-reperfusion on neutrophil 5-lipoxygenase activity and to define the role of leukotriene B4 (LTB4) in postischemic PMN infiltration into such composite tissue grafts. Anesthetized Yorkshire pigs underwent 6 h of rectus abdominis myocutaneous flap ischemia or sham ischemia, and LTB4 generation was measured in calcium ionophore-stimulated neutrophils isolated from the circulation. At 30 min of reperfusion, neutrophil generation of LTB4 increased from a baseline value of 31.0 +/- 6.8 to 98.5 +/- 5.1 ng/5 x 10(6) PMN (P < 0.01) and was significantly greater than those neutrophils isolated from animals subjected to sham ischemia and reperfusion (54.3 +/- 4.1 ng/5 x 10(6) PMN; P < 0.01). Pretreatment of animals with the LTB4-receptor antagonist, SC-41930 (n = 5), significantly attenuated reperfusion-associated 5-lipoxygenase activation (60.3 +/- 11.6 ng LTB4/5 x 10(6) PMN; P < 0.01), suggesting the presence of a positive feedback mechanism for eicosanoid biosynthesis.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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