Estrogen-mediated protection of the organotin-degrading strain Metarhizium robertsii against oxidative stress promoted by monobutyltin
| Autor: | Przemysław Bernat, Sylwia Różalska, Paulina Siewiera |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metarhizium Environmental Engineering Estrone Health Toxicology and Mutagenesis 010501 environmental sciences medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Superoxides medicine Organotin Compounds Environmental Chemistry Hydrogen peroxide 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species biology Estradiol Superoxide Superoxide Dismutase Public Health Environmental and Occupational Health Estrogens General Medicine General Chemistry Hydrogen Peroxide Pollution Oxidative Stress 030104 developmental biology Enzyme chemistry Biochemistry Catalase biology.protein Reactive Oxygen Species Oxidation-Reduction Oxidative stress |
| Zdroj: | Chemosphere. 185 |
| ISSN: | 1879-1298 |
| Popis: | Dibutyltin (DBT) is a global pollutant characterized by pro-oxidative properties. The fungal strain Metarhizium robertsii can eliminate high levels of DBT efficiently. In this study, induction of oxidative stress as well as its alleviation through the application of natural estrogens during the elimination of DBT by M. robertsii were evaluated. During the first 24 h of incubation, the initial concentration of DBT (20 mg l-1) was reduced to 3.1 mg l-1, with simultaneous formation of a major byproduct - monobutyltin (MBT). In the presence of estrone (E1) or 17β-estradiol (E2), the amounts of dibutyltin residues in the fungal cultures were found to be approximately 2-fold higher compared to cultures without estrogens, which was associated with the simultaneous utilization of the compounds by cytochrome P450 enzymes. On the other hand, MBT levels were approximately 2.5 times lower in the fungal cultures with the addition of one of the estrogens. MBT (not DBT) promotes the generation of O2-, H2O2, and NO at levels 65.89 ± 18.08, 4.04 ± 3.62, and 27.92 ± 1.95, respectively. Superoxide dismutase and catalase activities did not show any response of the M. robertsii strain against the overproduction of superoxide anion and hydrogen peroxide. Application of E1 as well as E2 ensured non-enzymatic defense against nitrosative and oxidative stress through scavenging of nitrogen and oxygen reactive species, and limited their levels from 1.5-fold to 21-fold, depending on the used estrogen. |
| Databáze: | OpenAIRE |
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