DNA authentication of brewery products: basic principles and methodological approaches
| Autor: | R. R. Vafin, Anastasia Ryabova, Lev Oganesyants, A.G. Galstyan, Vladislav Semipyatniy, Sergey Khurshudyan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine marker lcsh:TP368-456 Computer science Management science food and beverages DNA 01 natural sciences Authentication (law) 03 medical and health sciences lcsh:Food processing and manufacture 030104 developmental biology PCR 010608 biotechnology Alcoholic beverages malting barley authentication identification Hordeum vulgare Food Science |
| Zdroj: | Foods and Raw Materials, Vol 7, Iss 2, Pp 364-374 (2019) |
| ISSN: | 2310-9599 2308-4057 |
| Popis: | Beer DNA authentication is the process of authentication by identification of barley malt Hordeum vulgare or its substitutes, as well as hops and yeast. The method is based on molecular genetic analysis of residual quantities of nucleic acids extracted from the cellular debris of the final product. The aim of the study was to analyse scientific and methodical approaches to extraction of residual quantities of beer raw materials nucleic acids and beer DNA authentication for their later application in determining brewing products authenticity. The technological level discloses the method of DNA extraction from wines, modified for extraction of nucleic acids from beer samples. The method includes the following characteristic peculiarities: stage enzymatic hydrolysis of polysaccharides and polypeptides of dissolved lyophilisate, multiple sedimentation and resursuspension of nucleoproteid complex, RNA removal followed by DNA extraction by organic solvents, and additional DNA purification by magnetic particle adsorption. This review presents the analysis of genetic targets used as molecular markers for gene identification of malting barley varieties and beer DNA authentication. We also provided the interpretation of PCR analysis of Hordeum vulgare varieties and samples of commercial beer. Data on SSR- and SNP-markers of Hordeum vulgare nuclear DNA, used for barley varieties identification and potentially suitable for beer DNA authentication, are also presented. We also analysed genetic targets used in malting barley substitute detection, as well as hops and yeast identification in beer. Data on correlation of amplified DNA targets with beer quality indicators were systematised. |
| Databáze: | OpenAIRE |
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