Intrinsic properties of limb bud cells can be differentially reset
| Autor: | Patricia Saiz-Lopez, Matthew Towers, Kavitha Chinnaiya, Maria A. Ros |
|---|---|
| Přispěvatelé: | Consejo Superior de Investigaciones Científicas (España), Medical Research Council (UK), Ministerio de Economía y Competitividad (España), European Commission |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Limb Buds Chicks Proliferation Morphogenesis Chick Embryo Biology Animals Genetically Modified Avian Proteins 03 medical and health sciences Limb bud Proximo-distal Animals Regeneration Wings Animal Limb development Cell Lineage Progenitor cell Molecular Biology Embryonic Stem Cells Body Patterning Homeodomain Proteins Embryogenesis Gene Expression Regulation Developmental Cell Cycle Checkpoints Anatomy Embryonic stem cell Cell biology body regions 030104 developmental biology Hoxa13 embryonic structures Limb morphogenesis HOXA13 Research Article Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1477-9129 0950-1991 2014-5721 |
| Popis: | An intrinsic timing mechanism specifies the positional values of the zeugopod (i.e. radius/ulna) and then autopod (i.e. wrist/digits) segments during limb development. Here, we have addressed whether this timing mechanism ensures that patterning events occur only once by grafting GFP-expressing autopod progenitor cells to the earlier host signalling environment of zeugopod progenitor cells. We show by detecting Hoxa13 expression that early and late autopod progenitors fated for the wrist and phalanges, respectively, both contribute to the entire host autopod, indicating that the autopod positional value is irreversibly determined. We provide evidence that Hoxa13 provides an autopod-specific positional value that correctly allocates cells into the autopod, most likely through the control of cellsurface properties as shown by cell-cell sorting analyses. However, we demonstrate that only the earlier autopod cells can adopt the host proliferation rate to permit normal morphogenesis. Therefore, our findings reveal that the ability of embryonic cells to differentially reset their intrinsic behaviours confers robustness to limb morphogenesis. We speculate that this plasticity could be maintained beyond embryogenesis in limbs with regenerative capacity. The M.A.R. laboratory is supported by the Spanish Ministry of Economy and Innovation (Ministerio de Economía y Competitividad) (BFU2014-57216-P) and work in the M.T. laboratory is supported by the Medical Research Council (G1100295). P.S.-L. was supported by a CSIC JAE-Pre contract co-funded by the Fondo Social Europeo (FSE). |
| Databáze: | OpenAIRE |
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