Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans Delta 9-C18:1)
| Autor: | Ole N. Jensen, Clifford Young, Vladimiros Thoma, Carla Ferreri, Peter Højrup, Ida B. Thøgersen, Jan J. Enghild, Steffen Bak, Lone Nielsen, Toke P. Krogager |
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| Rok vydání: | 2012 |
| Předmět: |
Serum free
Trans fat Trans fatty acids Plasma biomarker Biophysics Oleic Acids Biochemistry Elaidic acid chemistry.chemical_compound Tandem Mass Spectrometry Stable isotope labeling by amino acids in cell culture Humans Carbon Radioisotopes RNA Messenger HepG2 cells Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Proteins Fatty acid Hep G2 Cells Dietary Fats Fold change Culture Media Amino acid Oleic acid Liver chemistry Electrophoresis Polyacrylamide Gel lipids (amino acids peptides and proteins) Stearic acid Biomarkers Stearic Acids Oleic Acid |
| Zdroj: | Journal of proteomics 75 (2012): 2685–2696. doi:10.1016/j.jprot.2012.03.023 info:cnr-pdr/source/autori:Krogager, Toke Peter; Nielsen, Lone Vendel; Bak, Steffen; Young, Clifford; Ferreri, Carla; Jensen, Ole Norregaard; Hojrup, Peter; Thoma, Vladimiros; Thogersen, Ida B.; Enghild, Jan J./titolo:Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans Delta 9-C18:1)/doi:10.1016%2Fj.jprot.2012.03.023/rivista:Journal of proteomics (Print)/anno:2012/pagina_da:2685/pagina_a:2696/intervallo_pagine:2685–2696/volume:75 Kroager, T P, Nielsen, L V, Bak, S, Young, C, Ferreri, C, Jensen, O N, Højrup, P, Thoma, V, Thøgersen, I B & Enghild, J J 2012, ' Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1) ', Journal of Proteomics, vol. 75, no. 9, pp. 2685-2696 . https://doi.org/10.1016/j.jprot.2012.03.023 Krogager, T P, Nielsen, L V, Bak, S, Young, C, Ferreri, C, Jensen, O N, Højrup, P, Thoma, V, Thøgersen, I & Enghild, J J 2012, ' Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1) ', Journal of Proteomics, vol. 75, no. 9, pp. 2685-96 . https://doi.org/10.1016/j.jprot.2012.03.023 |
| Popis: | Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 mu M elaidic acid (trans Delta 9-C18:1), oleic acid (cis Delta 9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value 1.3 fold change, P-value 1.3 fold change, P-value |
| Databáze: | OpenAIRE |
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