Direct sequencing of SSP-PCR-amplified cDNA to identify new alleles in the DR52-associated DRB1 group: identification of DRB1*1115, DRB1*1117 and DRB1*1319
| Autor: | Jennifer Ng, Fu-Meei Robbins, Carolyn Katovich Hurley, H. Yao, Robert J. Hartzman, T.F. Tang |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Immunology Biology Polymerase Chain Reaction Biochemistry Unrelated Donor Sequence Homology Nucleic Acid Complementary DNA Genetics Humans Immunology and Allergy Typing Allele Alleles Cell Line Transformed Sequence (medicine) Base Sequence Direct sequencing Genetic Variation HLA-DR Antigens General Medicine Group identification Single cell sequencing HLA-DRB1 Chains |
| Zdroj: | Tissue Antigens. 45:302-308 |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1995.tb02458.x |
| Popis: | Low and high resolution sequence specific oligonucleotide probe hybridization patterns were used to design an approach to direct sequencing of allele specific amplified cDNA. Several PCR amplifications were used to derive overlapping sequence fragments to define complete first domain sequences for a single allele. This method has been used to characterize three new DRB1 alleles in the DR52 family, DRB1*1115, DRB1* 1117, and DRB1*1319. All three alleles carry polymorphisms previously observed in other DRB alleles and underscore the importance of utilizing a directed sequencing approach for obtaining unambiguous typing results in matching for bone marrow transplantation between unrelated donor and recipient. |
| Databáze: | OpenAIRE |
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