Differential DNA Methylation in Prostate Tumors from Puerto Rican Men

Autor: Ryan Putney, Jarline Encarnación-Medina, Gilberto Ruiz-Deya, Carmen Ortiz-Sanchez, Julie Dutil, Jasreman Dhillon, Anders Berglund, Young-Chul Kim, Jaime Matta, Jong Y. Park
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Oncology
Male
Epigenesis
Genetic
lcsh:Chemistry
Prostate cancer
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
education.field_of_study
DNA methylation
General Medicine
Methylation
Middle Aged
prostate cancer
indolent prostate cancer
Computer Science Applications
Gene Expression Regulation
Neoplastic
CYP4F12
030220 oncology & carcinogenesis
Disease Progression
medicine.medical_specialty
DNA repair
Population
Biology
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Humans
Prostate tumors
Physical and Theoretical Chemistry
Gleason score
education
Molecular Biology
Gene
Aged
Neoplasm Staging
Gene Expression Profiling
ancestry
Organic Chemistry
Puerto Rico
Prostatic Neoplasms
medicine.disease
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
aggressive prostate cancer
CpG Islands
Neoplasm Grading
Hispanic/Latino
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 2
International Journal of Molecular Sciences, Vol 22, Iss 733, p 733 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22020733
Popis: In 2020, approximately 191,930 new prostate cancer (PCa) cases are estimated in the United States (US). Hispanic/Latinos (H/L) are the second largest racial/ethnic group in the US. This study aims to assess methylation patterns between aggressive and indolent PCa including DNA repair genes along with ancestry proportions. Prostate tumors classified as aggressive (n = 11) and indolent (n = 13) on the basis of the Gleason score were collected. Tumor and adjacent normal tissue were annotated on H&
E (Haemotoxylin and Eosin) slides and extracted by macro-dissection. Methylation patterns were assessed using the Illumina 850K DNA methylation platform. Raw data were processed using the Bioconductor package. Global ancestry proportions were estimated using ADMIXTURE (k = 3). One hundred eight genes including AOX1 were differentially methylated in tumor samples. Regarding the PCa aggressiveness, six hypermethylated genes (RREB1, FAM71F2, JMJD1C, COL5A3, RAE1, and GABRQ) and 11 hypomethylated genes (COL9A2, FAM179A, SLC17A2, PDE10A, PLEKHS1, TNNI2, OR51A4, RNF169, SPNS2, ADAMTSL5, and CYP4F12) were identified. Two significant differentially methylated DNA repair genes, JMJD1C and RNF169, were found. Ancestry proportion results for African, European, and Indigenous American were 24.1%, 64.2%, and 11.7%, respectively. The identification of DNA methylation patterns related to PCa in H/L men along with specific patterns related to aggressiveness and DNA repair constitutes a pivotal effort for the understanding of PCa in this population.
Databáze: OpenAIRE
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