Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein

Autor: Manuel A. Patarroyo, Manuel E. Patarroyo, Luis E. Rodriguez, Hernando Curtidor, David F. Plaza, Marisol Ocampo, Martha Forero
Rok vydání: 2008
Předmět:
Rv2969c
Rabbit
immunoelectron
Gene sequence
Monocyte
Oryctolagus cuniculus
Biochemistry
Bacterial proteins
Membrane proteins
Microscopy
Immunoelectron
Tuberculosis Vaccines
Peptide sequence
Phenylalanyltyrosylisoleucylvalylthreonylserylarginylaspartyl aspartyllysyllysylaspartylglycylvalylalanylglycylprolylglycylaspartylalanine
Priority journal
Microscopy
biology
Isoleucyllysylglutamylisoleucylvalylglycylaspartylvalylprolyl glycylisoleucylaspartylserylalanylalanylalanylthreonylalanyl threonylseryltyrosine
Antibodies
Bacterial
Polymerase chain reaction
Peptide
Rabbits
Tuberculosis vaccines
Lung alveolus epithelium
Human
Tuberculosis
Immunoelectron microscopy
Protein subunit
Molecular Sequence Data
Biophysics
Article
Antibodies
Cell Line
Amino acid sequence
Mycobacterium tuberculosis
Gene product
Bacterial Proteins
Molecular sequence data
medicine
Animals
Humans
bacterial
Amino Acid Sequence
Animal experiment
Molecular Biology
Antibody
Membrane Proteins
Cell Biology
Nonhuman
biology.organism_classification
medicine.disease
Molecular biology
Synthetic peptide
Human cell
Genes
Membrane protein
Genes
Bacterial
High activity binding peptide (habp)
Immunization
Cell line
Peptides
Mycobacterium tuberculosis-host cell interaction
Controlled study
Nucleotide sequence
Zdroj: Repositorio EdocUR-U. Rosario
Universidad del Rosario
instacron:Universidad del Rosario
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2008.05.157
Popis: Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.
Databáze: OpenAIRE
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