Carnitine Palmitoyltransferase in Patients with Cardiac Ischemia due to Atherosclerotic Coronary Artery Disease and in Patients with Idiopathic Dilated Cardiomyopathy
| Autor: | Iris Maurer, S. Zierz |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Cardiomyopathy Dilated Male endocrine system medicine.medical_specialty endocrine system diseases Biopsy Heart Ventricles medicine.medical_treatment Myocardial Ischemia Ischemia Cardiomyopathy Coronary Artery Disease Mitochondria Heart Coronary artery disease Internal medicine Idiopathic dilated cardiomyopathy medicine Humans heterocyclic compounds Pharmacology (medical) Carnitine Carnitine O-palmitoyltransferase neoplasms Heart transplantation Carnitine O-Palmitoyltransferase business.industry Cell Membrane Dilated cardiomyopathy Middle Aged medicine.disease Tissue Donors digestive system diseases Malonyl Coenzyme A Cardiology Heart Transplantation Cardiology and Cardiovascular Medicine business Biomarkers medicine.drug |
| Zdroj: | Cardiology. 88:258-263 |
| ISSN: | 1421-9751 0008-6312 |
| DOI: | 10.1159/000177340 |
| Popis: | Cardiac ischemia is associated with an impairment in long-chain fatty acid metabolism. We studied carnitine palmitoyltransferase (CPT) in left ventricular biopsies of 6 transplant recipients with ischemia due to atherosclerosis, 4 patients with dilated cardiomyopathy, and 5 donor hearts. Total CPT activity was not significantly different between the three groups (7.9 +/- 3; 6.7 +/- 2, and 8 +/- 3 nmol/min/mg noncollagenous protein). Residual CPT activity after inhibition by malonyl-CoA (0.4 mM) was 38 +/- 11, 36 +/- 5 and 38 +/- 7%. There were no difference in IC50 values. Residual CPT activity after the addition of the detergent Triton X-100 (0.5%) was 58 +/- 17, 54 +/- 2 and 50 +/- 8% (nonsignificant). Our results suggest that (i) total CPT activity and (ii) the sensitivity of the interaction of CPT I with its regulator malonyl-CoA are not affected by cardiac ischemia, and (iii) the ratio of CPT I to CPT II is not altered in cardiac ischemia. |
| Databáze: | OpenAIRE |
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