Novel Markers of the Metabolic Impact of Exogenous Retinoic Acid with A Focus on Acylcarnitines and Amino Acids
| Autor: | Andrea Arreguín, Joan Ribot, Andreu Palou, M L Bonet, Ondrej Kuda, Jan Kopecky |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Taurine Arginine Retinoic acid oxidación-reducción vitamin A lcsh:Chemistry chemistry.chemical_compound Mice 0302 clinical medicine acylcarnitines aminoácidos Leukocytes metabolómica targeted metabolomics retinoic acid Amino Acids tejido adiposo Acetylcarnitine lcsh:QH301-705.5 Spectroscopy lípidos chemistry.chemical_classification Chemistry General Medicine Lipids Computer Science Applications Amino acid Adipose Tissue Docosahexaenoic acid Metabolome tretinoina Oxidation-Reduction metaboloma medicine.drug Polyunsaturated fatty acid medicine.medical_specialty leucocitos 030209 endocrinology & metabolism Tretinoin Models Biological Article Catalysis Inorganic Chemistry 03 medical and health sciences Internal medicine Carnitine medicine Metabolomics Animals Physical and Theoretical Chemistry perfiles de expresión génica Molecular Biology Gene Expression Profiling Organic Chemistry Lipid Metabolism 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 Leukocytes Mononuclear animales metabolismo lipídico carnitina ratones |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 15 International Journal of Molecular Sciences, Vol 20, Iss 15, p 3640 (2019) |
| DOI: | 10.3390/ijms20153640 |
| Popis: | Treatment with all-trans retinoic acid (ATRA), the carboxylic form of vitamin A, lowers body weight in rodents by promoting oxidative metabolism in multiple tissues including white and brown adipose tissues. We aimed to identify novel markers of the metabolic impact of ATRA through targeted blood metabolomics analyses, with a focus on acylcarnitines and amino acids. Blood was obtained from mice treated with a high ATRA dose (50 mg/kg body weight/day, subcutaneous injection) or placebo (controls) during the 4 days preceding collection. LC-MS/MS analyses with a focus on acylcarnitines and amino acids were conducted on plasma and PBMC. Main results showed that, relative to controls, ATRA-treated mice had in plasma: increased levels of carnitine, acetylcarnitine, and longer acylcarnitine species decreased levels of citrulline, and increased global arginine bioavailability ratio for nitric oxide synthesis increased levels of creatine, taurine and docosahexaenoic acid and a decreased n-6/n-3 polyunsaturated fatty acids ratio. While some of these features likely reflect the stimulation of lipid mobilization and oxidation promoted by ATRA treatment systemically, other may also play a causal role underlying ATRA actions. The results connect ATRA to specific nutrition-modulated biochemical pathways, and suggest novel mechanisms of action of vitamin A-derived retinoic acid on metabolic health. |
| Databáze: | OpenAIRE |
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