In-gel digestion coupled with mass spectrometry (GeLC-MS/MS)-based salivary proteomic profiling of canine oral tumors

Autor: Suthathip Kittisenachai, Chanin Kalpravidh, Sekkarin Ploypetch, Narumon Phaonakrop, Anudep Rungsipipat, Sittiruk Roytrakul, Gunnaporn Suriyaphol, Kantinan Leetanasaksakul, Sirinun Pisamai
Rok vydání: 2019
Předmět:
Electrophoresis
Male
Proteomics
Saliva
In-gel digestion coupled with mass spectrometry (GeLC-MS/MS)
Protein tyrosine phosphatase
In-gel digestion
Mass spectrometry
Protein tyrosine phosphatase non-receptor type 5 (PTPN5)
Tumor protein p53 (p53)
03 medical and health sciences
0302 clinical medicine
Dogs
Western blot
Tandem Mass Spectrometry
Dog
Biomarkers
Tumor
Medicine
Animals
Dog Diseases
Periodontitis
Melanoma
030304 developmental biology
0303 health sciences
lcsh:Veterinary medicine
General Veterinary
medicine.diagnostic_test
business.industry
Proteomic Profiling
Oral tumors
General Medicine
medicine.disease
Protein Tyrosine Phosphatases
Non-Receptor
stomatognathic diseases
030220 oncology & carcinogenesis
Cancer research
Carcinoma
Squamous Cell
lcsh:SF600-1100
Female
Mouth Neoplasms
Tumor Suppressor Protein p53
business
Digestion
Research Article
Zdroj: BMC Veterinary Research
BMC Veterinary Research, Vol 16, Iss 1, Pp 1-17 (2020)
ISSN: 1746-6148
Popis: Background Various types of oral tumors, either benign or malignant, are commonly found in dogs. Since saliva directly contacts the tumors and saliva collection is non-invasive, easily accessible and cost effective, salivary biomarkers are practical to be used for the diagnosis and/or prognosis of these diseases. However, there is limited knowledge of protein expression in saliva for canine oral tumors. The present study aimed to investigate novel biomarkers from the salivary proteome of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP), using an in-gel digestion coupled with mass spectrometry (GeLC-MS/MS). The relationships between protein candidates and chemotherapy drugs were explored and the expression of potential biomarkers in saliva and tissues was verified by western blot analysis. Results For saliva samples, increased expression of protein tyrosine phosphatase non-receptor type 5 (PTPN5) was shown in all tumor groups compared with the CP group. Marked expression of PTPN5 was also observed in LOM and OSCC compared with that in BN and EOM. In addition, tumor protein p53 (p53), which appeared in the PTPN5–drug interactions, was exhibited to be expressed in all tumor groups compared with that in the CP group. For tissue samples, increased expression of p53 was shown in LOM compared with the control group. Conclusion PTPN5 and p53 were proposed to be potential salivary biomarkers of canine oral tumors.
Databáze: OpenAIRE
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