The study of variability and strain selection in Streptomyces atroolivaceus. I. UV light and nitrous acid as effective agents in the improvement of mithramycin production
| Autor: | Zdenko Vaněk, Margita Blumauerová, K. Stajner, D. A. S. Callieri |
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| Rok vydání: | 1974 |
| Předmět: |
Nitrous acid
Chromatography biology Strain (chemistry) Ultraviolet Rays Genetic Variation Nitrous Acid General Medicine Plicamycin Streptomyces atroolivaceus biology.organism_classification Microbiology Streptomyces chemistry.chemical_compound chemistry Auxotrophic mutant Radiation Genetics Biological Assay Irradiation Selection (genetic algorithm) Nitrites Bacillus subtilis Mutagens Nitrosoguanidines |
| Zdroj: | Folia microbiologica. 19(2) |
| ISSN: | 0015-5632 |
| Popis: | A seven-step selection procedure (repeated UV irradiation, single-step application of nitrous acid, and natural selection, following each mutagenic treatment) made it possible to increase production of mithramycin byStreptomyces atroolivaceus from 40–50 μg/ml to 680–830 μg/ml,i.e. roughly by 15 to 19-fold. The UV radiation was more effective when applying lower doses, yielding about 5% survival, 2.3% survival was obtained after the treatment with nitrous acid.N-Methyl-N′-nitro-N-nitrosoguanidine applied in a buffer pH 9.0) at doses yielding more than 99% killing was less effective than the two former mutagens. |
| Databáze: | OpenAIRE |
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