Mapping of a Linear Autoantigenic Epitope within the Human Thyroid Peroxidase Using Recombinant DNA Techniques
| Autor: | Heike Berthold, Wolfgang Northemann, Nicholas Hunt, Mirco Scanarini, Brigitte Frorath, Charles C. Abney |
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| Rok vydání: | 1992 |
| Předmět: |
endocrine system
DNA Recombinant Thyroid Gland Autoantigens Iodide Peroxidase Peptide Mapping Biochemistry Epitope law.invention Epitopes fluids and secretions Antigen law Thyroid peroxidase Complementary DNA medicine Humans Molecular Biology Autoantibodies biology Linear epitope cDNA library Thyroid Thyroiditis Autoimmune food and beverages hemic and immune systems DNA General Medicine Molecular biology Graves Disease medicine.anatomical_structure embryonic structures Recombinant DNA biology.protein |
| Zdroj: | The Journal of Biochemistry. 111:633-637 |
| ISSN: | 1756-2651 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a123810 |
| Popis: | Autoantibodies directed against the thyroid peroxidase (TPO), the thyroid microsomal antigen, are widely used to diagnose human autoimmune thyroid disease. A cloned 3.088 kb cDNA coding for the entire mature human TPO was isolated from a cDNA library derived from a pathological thyroid gland of a Graves' disease patient and used further to generate a so-called TPO epitope cDNA library in order to map linear autoantigenic epitopes involving a recombinant molecular biology approach. The TPO epitope cDNA library consisting of randomly fragmented cDNA sequences inserted in the expression vector pGEX-2T was expressed in Escherichia coli and screened with characterized anti-TPO autoantisera from Hashimoto's disease patients. All the sera were positively tested with a purified thyroid microsomal antigen fraction (TMA/TPO). Only about 1% of examined autoantisera were able to recognize bacterial expressed recombinant TPO representing sequential antigenic determinants. A corresponding autoantigenic epitope with 61 amino acids in length was located at the C-terminus of human TPO. |
| Databáze: | OpenAIRE |
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