Alpha satellite DNAs on chromosomes 10 and 12 are both members of the dimeric suprachromosomal subfamily, but display little identity at the nucleotide sequence level
| Autor: | Leendert H. J. Looijenga, Vincent T.H.B.M. Smit, Jw Oosterhuis, Peter Devilee, J.W. Wessels, P. Mollevanger |
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| Rok vydání: | 1992 |
| Předmět: |
Genetics
Chromosomes Human Pair 12 Base Sequence Chromosomes Human Pair 10 Molecular Sequence Data Nucleic Acid Hybridization Biology DNA Satellite Chromosome 17 (human) Blotting Southern Chromosome 16 Chromosome 4 Chromosome 3 Chromosome 18 Chromosome 19 Sequence Homology Nucleic Acid Humans Chromosome 21 Chromosome 22 Repetitive Sequences Nucleic Acid |
| Zdroj: | Genomics. 13(4) |
| ISSN: | 0888-7543 |
| Popis: | We have investigated the organization and complexity of alpha satellite DNA on chromosomes 10 and 12 by restriction endonuclease mapping, in situ hybridization (ISH), and DNA-sequencing methods. Alpha satellite DNA on both chromosomes displays a basic dimeric organization, revealed as a 6- and an 8-mer higher-order repeat (HOR) unit on chromosome 10 and as an 8-mer HOR on chromosome 12. While these HORs show complete chromosome specificity under high-stringency ISH conditions, they recognize an identical set of chromosomes under lower stringencies. At the nucleotide sequence level, both chromosome 10 HORs are 50% identical to the HOR on chromosome 12 and to all other alpha satellite DNA sequences from the in situ cross-hybridizing chromosomes, with the exception of chromosome 6. An 80% identity between chromosome 6- and chromosome 10-derived alphoid sequences was observed. These data suggest that the alphoid DNA on chromosomes 6 and 10 may represent a distinct subclass of the dimeric subfamily. These sequences are proposed to be present, along with the more typical dimeric alpha satellite sequences, on a number of different human chromosomes. |
| Databáze: | OpenAIRE |
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