Enterococcal Leucine-Rich Repeat-Containing Protein Involved in Virulence and Host Inflammatory Response
| Autor: | Samira Makhzami, Adriana Alberti, Sophie Brinster, Hélène Bierne, Pascale Serror, Maurizio Sanguinetti, Brunella Posteraro |
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| Přispěvatelé: | Bactéries Lactiques et Pathogènes Opportunistes (UBLO), Institut National de la Recherche Agronomique (INRA), Università cattolica del Sacro Cuore = Catholic University of the Sacred Heart [Roma] (Unicatt), Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unite 604, Institut Naltional de la Santé et de la Recherche Médicale, ProdInra, Migration, Università cattolica del Sacro Cuore [Roma] (Unicatt), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2007 |
| Předmět: |
Signal peptide
BACTERIE NOSOCOMIALE [SDV]Life Sciences [q-bio] Molecular Sequence Data Immunology Virulence Peritonitis Biology Leucine-rich repeat Microbiology Enterococcus faecalis Mice 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Leucine In vivo Animals Amino Acid Sequence Gene Peptide sequence Gram-Positive Bacterial Infections 030304 developmental biology Mice Inbred ICR 0303 health sciences Interleukin-6 030306 microbiology biology.organism_classification Molecular Pathogenesis 3. Good health [SDV] Life Sciences [q-bio] Disease Models Animal POUVOIR PATHOGENE Infectious Diseases chemistry Female Parasitology Peptidoglycan |
| Zdroj: | Infection and Immunity Infection and Immunity, 2007, 75 (9), pp.4463-4471. ⟨10.1128/IAI.00279-07⟩ Infection and Immunity, American Society for Microbiology, 2007, 75 (9), pp.4463-4471. ⟨10.1128/IAI.00279-07⟩ |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00279-07 |
| Popis: | Enterococcus faecalis is an important nosocomial pathogen associated with high morbidity and mortality for patients who are immunocompromised or who have severe underlying diseases. The E. faecalis genome encodes numerous surface-exposed proteins that may be involved in virulence. This work describes the characterization of the first internalin-like protein in E. faecalis , ElrA, belonging to the recently identified WxL family of surface proteins. ElrA contains an N-terminal signal peptide for export, a leucine-rich repeat domain that may interact with host cells, and a C-terminal WxL domain that interacts with the peptidoglycan. Disruption of the elrA gene significantly attenuates bacterial virulence in a mouse peritonitis model. The elrA deletion mutant also displays a defect in infection of host macrophages and a decreased interleukin-6 response in vivo. Finally, elrA expression is induced in vivo. Altogether, these results demonstrate a role for ElrA in the E. faecalis infectious process in vivo and suggest that this surface protein may contribute to E. faecalis virulence by stimulating the host inflammatory response. |
| Databáze: | OpenAIRE |
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