Differential Frequency of CD8+ T Cell Subsets in Multiple Sclerosis Patients with Various Clinical Patterns
| Autor: | Mohammad Ali Sahraian, Maryam Izad, Rozita Doosti, Masoumeh Beheshti, Zahra Salehi, Ehsan Janzamin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Physiology Cellular differentiation lcsh:Medicine Pathogenesis CD8-Positive T-Lymphocytes Pathology and Laboratory Medicine Gastroenterology 0302 clinical medicine Immune Physiology Cellular types Cytotoxic T cell lcsh:Science Innate Immune System Multidisciplinary medicine.diagnostic_test Immune cells Healthy subjects Neurodegenerative Diseases Cell Differentiation Hematology Body Fluids Blood Neurology White blood cells Cytokines Female Anatomy Research Article Adult Cell biology Blood cells medicine.medical_specialty Multiple Sclerosis Patients Immunology T cells Cytotoxic T cells Autoimmune Diseases Flow cytometry 03 medical and health sciences Internal medicine Remission phase medicine Humans Medicine and health sciences Biology and life sciences business.industry Multiple sclerosis lcsh:R Molecular Development medicine.disease Demyelinating Disorders Health Care Animal cells Immune System Clinical Immunology lcsh:Q Clinical Medicine business 030217 neurology & neurosurgery CD8 Developmental Biology 030215 immunology |
| Zdroj: | PLoS ONE, Vol 11, Iss 7, p e0159565 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Recent evidence points to a pathogenic role for CD8+ cytotoxic T (Tc) cells in Multiple sclerosis (MS). Based on cytokine profile, Tc cells can be divided into different subsets: IFN-γ (Tc1), IL-4 (Tc2), IL-10 (Tc10), IL-17 (Tc17), IL-21 (Tc21), IL-22 (Tc22) and TNF-α producing cells. In this study we evaluated the frequency of Tc cell subsets and the serum level of Tc17 differentiation cytokines in MS patients with different clinical patterns. We analyzed Tc cell subsets percentage in peripheral blood of relapsing-remitting (RRMS) (n = 28), secondary-progressive (SPMS) (n = 10) and primary-progressive (PPMS) (n = 4) MS patients in comparison to healthy controls (n = 15) using flow cytometry. Serum level of TGF-β, IL-6 and IL-23 were measured by ELISA. We showed elevated levels of Tc1 and Tc17 cells in SPMS and RRMS patients in relapse phase, respectively (P = 0.04). Interestingly, the percentage of TNF-α producing CD8+ T cells in relapse and remission phase of RRMS and SPMS patients were higher than controls (P = 0.01, P = 0.004, P = 0.01, respectively) and Tc21 increased in remission phase of RRMS compared to SPMS (P = 0.03). We also found higher frequency of CD8+ IFN-γ+ TNF-α+ IL-17+ T cells in relapse phase of RRMS compared to remission phase, SPMS patients and controls (P = 0.01, P = 0.004 and P = 0.02, respectively). TGF- β increased in sera of RRMS patients in remission phase (P = 0.03) and SPMS (P = 0.05) compared to healthy subjects. Increased level of Tc17 and CD8+ IFN-γ+ TNF-α+ IL-17+ T cells in relapse phase highlights the critical role of IL-17 in RRMS pathogenesis. |
| Databáze: | OpenAIRE |
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