Effect of hypoxic-ischemic injury on serine palmitoyltransferase activity in the developing rat brain
| Autor: | Masaki Daigo, Yohei Kitamura, Yasuhiro Arai, Yuichiro Yamashiro, Masaharu Hayashi, Toshiaki Shimizu, Kyoichi Oshida |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Ceramide
Proteolipid protein 1 Central nervous system Serine C-Palmitoyltransferase Pharmacology Pathology and Forensic Medicine chemistry.chemical_compound Microsomes medicine Animals Rats Wistar Molecular Biology Myelin Sheath Hypoxic ischemic Sphingolipids biology Serine C-palmitoyltransferase Brain Cell Biology General Medicine Rat brain Sphingolipid Immunohistochemistry Myelin basic protein Animals Suckling Rats carbohydrates (lipids) Disease Models Animal medicine.anatomical_structure Biochemistry chemistry Hypoxia-Ischemia Brain biology.protein lipids (amino acids peptides and proteins) |
| Zdroj: | Pathobiology : journal of immunopathology, molecular and cellular biology. 75(6) |
| ISSN: | 1423-0291 |
| Popis: | Background: Sphingolipid metabolism is strongly associated with central nervous system myelination. Ceramide is the most active of the sphingolipid metabolites. On the basis of ceramide biosynthesis indicated by serine palmitoyltransferase activity and cerebroside generated by ceramide, the evaluation of serine palmitoyltransferase activity in developing brains or hypoxic-ischemic damaged brains is worthwhile. Methods: Using a scintillation counter, we assessed serine palmitoyltransferase activity, a rate-limiting enzyme of sphingolipid metabolism, in the brains of developing rats and compared the activity with hypoxic-ischemic brains, using the method of Rice on postnatal day 7 (P7). Results: In the control groups, serine palmitoyltransferase activity was detected in the microsomal fraction of whole brain homogenates from P4, which was earlier than the initial expression of myelin-specific proteins such as myelin basic protein and proteolipid protein on immunochemistry. Serine palmitoyltransferase activity increased along with development on P8, P10, P14 and P21. However, hypoxic-ischemic brains showed lower serine palmitoyltransferase activity than control brains on P8, P10, P14 and P21. Conclusions: These results suggest that increase in serine palmitoyltransferase activity before myelin-specific protein expression may be an initial step in myelinogenesis and a decline in serine palmitoyltransferase activity in hypoxic-ischemic brains may be one of the major causes of delayed myelination. |
| Databáze: | OpenAIRE |
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