| Autor: |
Endres, Kristina, Reinhardt, Sven, Geladaris, Anastasia, Knies, Julia, Grimm, Marcus, Hartmann, Tobias, Schmitt, Ulrich |
| Rok vydání: |
2016 |
| DOI: |
10.6084/m9.figshare.c.3621401_d2 |
| Popis: |
Additional file 1: Analysis of A-beta 42 peptides from acute transnasally treated mice. An exemplary wild type animal was treated with TAMRA-labeled A-beta 42 peptides and a control mouse with solvent administered to the nose (see Figure 2B). Brains were dissected 1 h later and homogenized as described. Extraction supernatant was subjected to human A-beta 42 ELISA (IBL) as recommended by the vendor. Values obtained for A-beta 42 containing slices were corrected for background values obtained from slices of solvent-control. Note that signal intensities for the six slices deviate from the fluorescence-based A-beta 42 measurement. This might indicate a partial liberation of the fluorophore from the peptide or be due to interference of tissue contents with the two different measurement techniques. Nevertheless, human A-beta 42 peptides were detectable throughout all of the six slices upon intranasal application also by peptide-directed ELISA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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