Rapid quantification of viable fungi in hospital environments: analysis of air and surface samples using solid-phase cytometry

Autor: P. Le Cann, Jean-Pierre Gangneux, D. Meheust
Přispěvatelé: Laboratoire d'étude et de recherche en environnement et santé (LERES), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Rok vydání: 2013
Předmět:
MESH: Fungi
Microbiology (medical)
Veterinary medicine
Air sampling
Fungal contamination
Air Microbiology
Colony Count
Microbial
Air sampler
010501 environmental sciences
Biology
01 natural sciences
Microbiology
Hospital
03 medical and health sciences
MESH: Air Microbiology
Environmental Microbiology
Humans
Malt extract agar
MESH: Colony Count
Microbial
Image Cytometry
MESH: Microbial Viability
0105 earth and related environmental sciences
Viable fungi
0303 health sciences
Microbial Viability
MESH: Humans
030306 microbiology
Environmental surveillance
Solid-phase cytometry
fungi
Significant difference
Fungi
MESH: Environmental Microbiology
General Medicine
MESH: Hospitals
Hospitals
3. Good health
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infectious Diseases
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Infectious risk
Cytometry
MESH: Image Cytometry
Zdroj: Journal of Hospital Infection
Journal of Hospital Infection, WB Saunders, 2013, 83 (2), pp.122-6. ⟨10.1016/j.jhin.2012.10.004⟩
Journal of Hospital Infection, 2013, 83 (2), pp.122-6. ⟨10.1016/j.jhin.2012.10.004⟩
ISSN: 0195-6701
1532-2939
DOI: 10.1016/j.jhin.2012.10.004
Popis: International audience; BACKGROUND: Environmental surveillance is important in high-risk areas of hospitals to prevent fungal infections in immunosuppressed patients. Conventional culture methods for enumerating environmental fungi are time-consuming. AIM: In this field study, a solid-phase cytometry technique (SPC) and a more conventional culture-based method to quantify fungal contamination of hospital air and surface samples were compared. METHODS: For the air sampling, a liquid cyclone air sampler was used with a flow rate of 300 L/min for 10 min in each of four hospital locations. Surface swabbing was done in two locations, with two different swab types. Samples from all areas were processed by SPC and by culture on malt extract agar. FINDINGS: The mean airborne concentrations of viable fungi determined by SPC were about 1.5-fold higher than the mean concentrations obtained with the culture-based method. These differences for air samples were significant in three hospital environments. No significant difference was observed for surface samples between the two swab types and between the two analytical methods. One of the prominent advantages of SPC was its rapidity in comparison with the culture-based method (5 h versus 5 days). CONCLUSION: In this study, we showed that SPC allows for rapid monitoring of viable fungi in hospital environments. SPC can thus be used to provide an early warning and a rapid implementation of corrective measures. Viable fungi detection may be an important tool to assess infectious risk in wards with immunosuppressed patients.
Databáze: OpenAIRE
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