Optimization extraction, structural features and antitumor activity of polysaccharides from Z. jujuba cv. Ruoqiangzao seeds
| Autor: | Yang Dajian, Hongjun Tan, Yang Luo, Hong Li, Yong Zhan, Yongde Wang, Zhen Wu, Gang Chen, Yong Yang |
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| Rok vydání: | 2019 |
| Předmět: |
Arabinose
Chemical Phenomena Rhamnose Cell Survival Apoptosis 02 engineering and technology Xylose Polysaccharide Biochemistry HeLa 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship Structural Biology Polysaccharides Cell Line Tumor Humans Response surface methodology Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography biology Spectrum Analysis Extraction (chemistry) Ziziphus General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Antineoplastic Agents Phytogenic chemistry Galactose Seeds Thermogravimetry 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 135 |
| ISSN: | 1879-0003 |
| Popis: | As a discarded byproduct of jujube (Z. jujuba), its seeds contain various biological components. Response surface methodology was used to optimize ultrasound-assisted heating extraction conditions of polysaccharides from Z. jujuba cv. Ruoqiangzao seeds (ZJSPs). The optimal parameters were as follows: temperature 83.1 °C, time 100 min, ultrasonic power 140 W, and water-material ratio 33.5 mL/g, allowed a maximum yield of 1.97%. One main fraction (ZJSPs-1) was successfully purified by ion-exchange and gel-permeation chromatography. With a molecular weight of 342 kDa, ZJSPs-1 was composed of arabinose, glucose, xylose, galactose and rhamnose. The presence of pyranose-form sugars as well as both α- and β-configurations was validated by FT-IR and 1H NMR. TG/DSC indicated that ZJSPs-1 has a favorable thermal stability. XRD suggested that ZJSPs-1 exhibited both crystalline and amorphous portions. ZJSPs-1 was in aggregates of homogenous-dense honeycomb like structures observed by SEM and AFM. ZJSPs-1 possessed antitumor activities against HeLa cells in a dose- and time-dependent manner at 24 and 48 h with IC50 of 164.6 and 87.1 μg/mL, respectively. Fluorescent microscopic and flow cytometry revealed that ZJSPs-1 inhibited the proliferation of HeLa cells through apoptosis. Z. jujuba seeds are good sources for antitumor polysaccharides in pharmaceutical and food industries. |
| Databáze: | OpenAIRE |
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