A comparison of silver staining methods for detecting proteins in ultrathin polyacrylamide gels on support film after isoelectric focusing
| Autor: | John K. Tomkins, Lynda A. Butcher |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Chromatography
Silver Staining and Labeling Isoelectric focusing Polyacrylamide Biophysics Proteins Cell Biology Biochemistry Staining Silver stain Electrophoresis chemistry.chemical_compound chemistry Rosaniline Dyes Electrophoresis Polyacrylamide Gel Isoelectric Focusing Coloring Agents Molecular Biology |
| Zdroj: | Analytical biochemistry. 148(2) |
| ISSN: | 0003-2697 |
| Popis: | The application of silver staining methods to the detection of proteins on ultrathin isoelectric focusing gel systems requires the optimization of many steps in the procedure in order to obtain reproducible staining of proteins with acceptable levels of background. Three different methods which have been reported for detecting proteins by silver staining in sodium dodecyl sulfate-polyacrylamide gel systems were investigated. A major problem with staining ultrathin isoelectric focusing gels was found to be surface staining that was associated with gels cast on support films. A modification of the method of Poehling and Neuhoff ( H.-M. Poehling and V. Neuhoff, 1981 , Electrophoresis 2 , 141–147) was found to give the best results. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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