FKBP51 immunohistochemical expression: A new prognostic biomarker for OSCC?
| Autor: | Maria Fiammetta Romano, Massimo Mascolo, Gennaro Ilardi, Luigi Califano, Virginia Napolitano, Simona Romano, Loredana Postiglione, Daniela Russo, Giovanni Orabona Dell’Aversana, Stefania Staibano, Angela Celetti, Pierpaolo Di Lorenzo, Silvia Varricchio, Francesco Merolla, F Astarita |
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| Přispěvatelé: | Russo, Daniela, Merolla, Francesco, Mascolo, Massimo, Ilardi, Gennaro, Romano, Simona, Varricchio, Silvia, Napolitano, Virginia, Celetti, Angela, Postiglione, Loredana, DI LORENZO, Pierpaolo, Califano, Luigi, DELL'AVERSANA ORABONA, Giovanni, Astarita, Fabio, Romano, MARIA FIAMMETTA, Staibano, Stefania |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Male Pathology Bayes theorem medicine.medical_treatment Gene Expression Kaplan-Meier Estimate Pathogenesis lcsh:Chemistry 0302 clinical medicine Squamous cell carcinoma lcsh:QH301-705.5 Spectroscopy Aged 80 and over Human papillomavirus 16 Oral cancer Computer Science Applications1707 Computer Vision and Pattern Recognition General Medicine Middle Aged FKBP51 Immunotherapy Prognosis Catalysis Molecular Biology Physical and Theoretical Chemistry Organic Chemistry Inorganic Chemistry Immunohistochemistry Computer Science Applications medicine.anatomical_structure 030220 oncology & carcinogenesis Lymphatic Metastasis Carcinoma Squamous Cell Female Mouth Neoplasms prognosi Adult medicine.medical_specialty In situ hybridization Article Tacrolimus Binding Proteins 03 medical and health sciences Immune system Tongue Internal medicine medicine Biomarkers Tumor Humans Cyclin-Dependent Kinase Inhibitor p16 Aged Neoplasm Staging Chemotherapy business.industry Radiation therapy stomatognathic diseases oral cancer prognosis immunotherapy squamous cell carcinoma 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Cutaneous melanoma Neoplasm Grading business |
| Zdroj: | International journal of molecular sciences 18 (2017). doi:10.3390/ijms18020443 info:cnr-pdr/source/autori:Daniela R.; Francesco M.; Mascolo M.; Ilardi G.; Romano S.; Varricchio S.; Napolitano V.; Celetti A.; Postiglione L.; Di Lorenzo P.P.; Califano L.; Dell'Aversana G.O.; Astarita F.; Romano M.F.; Staibano S./titolo:FKBP51 immunohistochemical expression: A new prognostic biomarker for OSCC?/doi:10.3390%2Fijms18020443/rivista:International journal of molecular sciences (Print)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:18 International Journal of Molecular Sciences, Vol 18, Iss 2, p 443 (2017) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 18; Issue 2; Pages: 443 |
| DOI: | 10.3390/ijms18020443 |
| Popis: | Up-to-date, several molecular markers of prognosis have been studied in Oral Squamous Cell Carcinoma (OSCC), but none entered in the clinical setting. Therapy of OSCC tumors mainly relies on surgery, radiotherapy and partially on chemotherapy; there is an urgent need for biomarkers able to better stratify OSCC patients’ risk to address targeted therapeutic strategies. The role of immune response in the pathogenesis and biological behavior of OSCC has been investigated by several authors, and promising results have been obtained with immune checkpoint inhibitors. We already investigated the role of the immune modulator FK506-binding protein 51 (FKBP51), a FK506-binding immunophilin, in cutaneous melanoma biology, and its expression in several human solid tumors. In the present study, we aimed to assess the value of FKBP51 expression in OSCC tumor cells as a marker of outcome. We collected clinical data from 72 patients who underwent surgery for Squamous Cell Carcinoma (SCC) of the tongue, floor, lips and palate. FKBP51 expression was assessed by immunohistochemistry on paraffin-embedded tumor tissues. In addition, we evaluated the human papillomavirus (HPV) status of primary tumors by immunohistochemistry, viral subtyping and In Situ Hybridization (ISH) assay. We found that high FKBP51-expressing tumors characterized the OSCCs with the worst prognosis: the high immunohistochemical expression of FKBP51 associated with death occurring within five years from the diagnosis with a sensitivity of 88.46% and a specificity of 91.67%. The estimated positive predictive value of the test was 88.45% and negative predictive value 91.67%. We tested FKBP51 mRNA presence, by RT-PCR assay, in a selected series of OSCC tumors, and we found that mRNA correlated well to the protein expression and to the clinical outcome. Applying the Bayes formula, we estimated an 88% probability of dying within five years from the diagnosis of OSCC patients with a high FKBP51 immunohistochemical (IHC) test result (>51% of FKBP51 positive tumor cells). On the basis of our analysis, we propose tumor tissue expression of FKBP51 protein as a reliable prognostic marker for OSCC tumors. |
| Databáze: | OpenAIRE |
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