The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis
| Autor: | Riccardo Angelini, Paraskevi Tavladoraki, Renato A. Rodrigues-Pousada, Ilaria Fraudentali, Andrea Carucci, Sandip A. Ghuge, Alessandra Cona |
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| Přispěvatelé: | Fraudentali, I., Ghuge, S. A., Carucci, A., Tavladoraki, P., Angelini, R., Cona, A., Rodrigues-Pousada, R. A. |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Polyamine H2O2 polyamines Mutant Plant Science Genetically modified crops 01 natural sciences Article Stomatal closure 03 medical and health sciences chemistry.chemical_compound lcsh:Botany Guard cell Arabidopsis copper amine oxidases H2O2 ROS polyamines ABA stomatal closure Arabidopsis thaliana Hydrogen peroxide Abscisic acid Ecology Evolution Behavior and Systematics Ecology biology Chemistry fungi food and beverages ROS biology.organism_classification copper amine oxidases stomatal closure lcsh:QK1-989 Cell biology 030104 developmental biology ABA Copper amine oxidase Amine gas treating 010606 plant biology & botany |
| Zdroj: | Plants, Vol 8, Iss 6, p 183 (2019) Plants Volume 8 Issue 6 |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants8060183 |
| Popis: | Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAO&delta (AtCuAO&delta At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAO&delta is up-regulated by ABA and that two Atcuao&delta T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAO&delta over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2O2 production compared to WT plants. Our data suggest that AtCuAO&delta is involved in the H2O2 production related to ABA-induced stomatal closure. |
| Databáze: | OpenAIRE |
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