Antiproliferative and apoptosis-inducing effect of common Tunisian date seed (var. Korkobbi and Arechti) phytochemical-rich methanolic extract
| Autor: | Guido Flamini, Amira Thouri, Lorena Canuti, Aicha Jelled, Livia La Barbera, Maria Rosa Ciriolo, Lotfi Achour, Rolando Vegliante |
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| Rok vydání: | 2018 |
| Předmět: |
Date seed
Antioxidant Phytochemical compounds DPPH Cell Survival Health Toxicology and Mutagenesis medicine.medical_treatment Phytochemicals Apoptosis 010501 environmental sciences 01 natural sciences Antioxidants HeLa chemistry.chemical_compound TBARS medicine Environmental Chemistry Humans Settore BIO/10 0105 earth and related environmental sciences Cell Proliferation Antiproliferative effect ABTS biology Traditional medicine Chemistry Plant Extracts Methanol Phoeniceae General Medicine Hep G2 Cells biology.organism_classification Pollution Phytochemical Antioxidant activities Seeds Growth inhibition HeLa Cells |
| Zdroj: | Environmental science and pollution research international. 26(36) |
| ISSN: | 1614-7499 |
| Popis: | In this study, the potential of date seed extracts to induce growth inhibition and apoptosis in HepG2 and HeLa cells was investigated. Analysis of the phytochemical compound content of the two Tunisian minor date seed extracts named Arechti and Korkobbi was determined. Moreover, their antioxidant properties are assessed through different assays including DPPH, ABTS, FRAP, TBARS, and phosphomolybdenum methods. Whereas, the cytotoxic effect was evaluated and apoptosis induction was confirmed by western blot technique (caspase-9, caspase-3, and PARP-1). The results proved the richness in phytochemical compounds of these by-products which explains the high in vitro antioxidant activity and the antiproliferative effects of both seed extracts. Additionally, the decrease in total PARP-1, procaspase-3 levels, and the increase of cleaved caspase-9 revealed the apoptotic effect of date seed extracts. These results collectively illustrate the potential of date seed extracts to induce growth inhibition and apoptosis in HepG2 and HeLa cells thanks to its phytochemical richness. |
| Databáze: | OpenAIRE |
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