Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models
| Autor: | Daniel M. Lachance, Naisi Li, Neelendu Dey, Sean T. Koester |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Science (General) ved/biology.organism_classification_rank.species Motility Biology Microbiology General Biochemistry Genetics and Molecular Biology Intracolonic Feces Mice Q1-390 Model Organisms Protocol Animals Germ-Free Life Gastrointestinal Transit Model organism General Immunology and Microbiology Gut motility ved/biology General Neuroscience digestive oral and skin physiology Cell biology Gastrointestinal Tract Disease Models Animal Female Biomarkers |
| Zdroj: | STAR Protocols, Vol 2, Iss 4, Pp 100938-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021). Graphical abstract Highlights • Visual and fluorescent markers can characterize gut transit with spatial resolution • Identify metabolites’ effects on colonic transit via intra-colonic metabolite infusion • Transit assays can interfere with each other, so an interval between each use is required Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. |
| Databáze: | OpenAIRE |
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