Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching
| Autor: | Hangyuan Guo, Hui Lin, Hangqi Luo, Fukang Xu, Jie Zhang, Jufang Chi, Sunlei Pan, Tingjuan Ni, Liping Meng, Feidan Gao, Guomei Ru |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Vascular smooth muscle Calponin Phenotypic switching Hyperhomocysteinemia Activating transcription factor Muscle Smooth Vascular Mice 03 medical and health sciences Animals Medicine Cells Cultured Cell Proliferation Mice Knockout biology ATF6 business.industry Endoplasmic reticulum Membrane Proteins Atherosclerosis musculoskeletal system Molecular biology Phenotype 030104 developmental biology Gene Knockdown Techniques LDL receptor cardiovascular system Unfolded protein response biology.protein Cardiology and Cardiovascular Medicine business Genes Switch |
| Zdroj: | International Journal of Cardiology. 269:242-249 |
| ISSN: | 0167-5273 |
| DOI: | 10.1016/j.ijcard.2018.07.043 |
| Popis: | Background Phenotypic switching of vascular smooth muscle cells (VSMCs) plays a key role in atherosclerosis. We aimed to investigate whether Homocysteine-responsive endoplasmic reticulum protein (Herp) was involved in VSMC phenotypic switching and affected atheroprogression. Methods To assess the role of Herp in homocysteine (Hcy)-associated atherosclerosis, Herp −/− and LDLR −/− double knockout mice were generated and fed with a high methionine diet (HMD) to induce Hyperhomocysteinemia (HHcy). Atherosclerotic lesions, cholesterol homeostasis, endoplasmic reticulum (ER) stress activation, and the phenotype of VSMCs were assessed in vivo . We used siRNAs to knockdown Herp in cultured VSMCs to further validate our findings in vitro . Results HMD significantly activated the activating transcription factor 6 (ATF6)/Herp arm of ER stress in LDLR −/− mice, and induced the phenotypic switch of VSMCs, with the loss of contractile proteins (SMA and calponin) and an increase of OPN protein. Herp −/− /LDLR −/− mice developed reduced atherosclerotic lesions in the aortic sinus and the whole aorta when compared with LDLR −/− mice. However, Herp deficiency had no effect on diet-induced HHcy and hyperlipidemia. Inhibition of VSMC phenotypic switching, decreased proliferation and collagen accumulation were observed in Herp −/− /LDLR −/− mice when compared with LDLR −/− mice. In vitro experiments demonstrated that Hcy caused VSMC phenotypic switching, promoted cell proliferation and migration; this was reversed by Herp depletion. We achieved similar results via inhibition of ER stress using 4-phenylbutyric-acid (4-PBA) in Hcy-treated VSMCs. Conclusion Herp deficiency inhibits the phenotypic switch of VSMCs and the development of atherosclerosis, thus providing novel insights into the role of Herp in atherogenesis. |
| Databáze: | OpenAIRE |
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