[Simultaneous determination of quercitrin, phloridzin and 3-hydroxyl phloridzin in leaves of Malus halliana by ultrasound-assisted ionic liquid-reversed phase liquid chromatography]
| Autor: | Xin Shao, Yong-Xin Huang, Lin Zhao, Cheng-Can Lu, Wenyi Kang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Phytochemicals
Ionic Liquids Ultrasound assisted High-performance liquid chromatography chemistry.chemical_compound 0404 agricultural biotechnology Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Acetonitrile Chromatography High Pressure Liquid Chromatography Reverse-Phase Chromatography biology 04 agricultural and veterinary sciences Reversed-phase chromatography biology.organism_classification 040401 food science Quercitrin Plant Leaves Phlorhizin Complementary and alternative medicine chemistry Malus Ionic liquid Quercetin Quantitative analysis (chemistry) Malus halliana |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 41(11) |
| ISSN: | 1001-5302 |
| Popis: | To establish a method for the simultaneous determination of phloridzin, 3-hydroxy phloridzin and quercitrin in leaves of Malus halliana by ultrasonic-assisted ionic liquid coupled with RP-HPLC. An Agilent TC-C₁₈ (4.6 mm×250 mm, 5 μm) column was used, with the mobile phase of acetonitrile and 1% phosphoric acid-water (20∶80) by gradient elution at the detection wavelength of 270 nm. The flow rate was 0.8 mL•min⁻¹, and chromatographic column temperature was controlled at the room temperature. Under the optimized conditions, the linear ranges for phloridzin, 3-hydroxy phloridzin and quercitrin were 0.9-112.5 μg (r = 0.999 6), 0.093 2-11.65 μg (r = 0.999 1) and 0.097 2-12.15 μg (r = 0.999 8), respectively. The average recoveries of the three constituents were 99.35%, 98.80% and 98.19%, respectively. The method was environmental friendly, rapid, accurate and highly reproducible, and so suitable for the quantitative analysis of phloridzin, 3-hydroxy phloridzin and quercitrin in leaves of M. halliana. |
| Databáze: | OpenAIRE |
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