Longitudinal characteristics of lymphocyte responses and cytokine profiles in the peripheral blood of SARS-CoV-2 infected patients
| Autor: | Fengqin Zhou, Jun Wang, Hua Wang, Zhihong Weng, Xiaobei Wang, Helong Zhou, Ruxia Zhou, Cheng Peng, Jing Liu, Hui Shen, Baoju Wang, Shengsong He, Ulf Dittmer, Qiaoxia Tong, Chunxia Guo, Wei Li, Mengji Lu, Chunrong Han, Jianao Zhang, Ling Xu, Huabing Zhu, Ran Pang, Pian Ye, Xin Zheng, Yinping Lu, Yong-Wen He, Boyun Liang, Li Zhang, Ting Liu, Bin Zhu, Jianhua Yi, Lijuan Xiong, Sihong Lu, Qian Zhang, Jia Liu, Weixian Wang, Ping Wei, Jinzhuo Luo, Ling Li, Sumeng Li, Lu Wang, Wenqing Zhou, Jin Tian, Dongliang Yang, Xiliu Chen, Lei Zhao, Shenghua Jie, Yu Hu, Jinghong Yao, Jun Wu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Research paper Inflammatory cytokine COVID-19 lymphopenia inflammatory cytokine SARS-CoV-2 Coronavirus Lymphocyte medicine.medical_treatment T cell Pneumonia Viral Medizin lcsh:Medicine Disease General Biochemistry Genetics and Molecular Biology Proinflammatory cytokine 03 medical and health sciences Betacoronavirus 0302 clinical medicine Lymphopenia medicine Humans Lymphocytes Pandemics lcsh:R5-920 business.industry lcsh:R General Medicine medicine.disease Comorbidity Cytokine release syndrome 030104 developmental biology medicine.anatomical_structure Cytokine Severe acute respiratory syndrome-related coronavirus 030220 oncology & carcinogenesis Immunology Cytokines lcsh:Medicine (General) business Coronavirus Infections CD8 |
| Zdroj: | EBioMedicine EBioMedicine, 55:102763 EBioMedicine, Vol 55, Iss, Pp-(2020) |
| Popis: | Background The dynamic changes of lymphocyte subsets and cytokines profiles of patients with novel coronavirus disease (COVID-19) and their correlation with the disease severity remain unclear. Methods Peripheral blood samples were longitudinally collected from 40 confirmed COVID-19 patients and examined for lymphocyte subsets by flow cytometry and cytokine profiles by specific immunoassays. Findings Of the 40 COVID-19 patients enrolled, 13 severe cases showed significant and sustained decreases in lymphocyte counts [0·6 (0·6-0·8)] but increases in neutrophil counts [4·7 (3·6-5·8)] than 27 mild cases [1.1 (0·8-1·4); 2·0 (1·5-2·9)]. Further analysis demonstrated significant decreases in the counts of T cells, especially CD8+ T cells, as well as increases in IL-6, IL-10, IL-2 and IFN-γ levels in the peripheral blood in the severe cases compared to those in the mild cases. T cell counts and cytokine levels in severe COVID-19 patients who survived the disease gradually recovered at later time points to levels that were comparable to those of the mild cases. Moreover, the neutrophil-to-lymphocyte ratio (NLR) (AUC=0·93) and neutrophil-to-CD8+ T cell ratio (N8R) (AUC =0·94) were identified as powerful prognostic factors affecting the prognosis for severe COVID-19. Interpretation The degree of lymphopenia and a proinflammatory cytokine storm is higher in severe COVID-19 patients than in mild cases, and is associated with the disease severity. N8R and NLR may serve as a useful prognostic factor for early identification of severe COVID-19 cases. Funding The National Natural Science Foundation of China, the National Science and Technology Major Project, the Health Commission of Hubei Province, Huazhong University of Science and Technology, and the Medical Faculty of the University of Duisburg-Essen and Stiftung Universitaetsmedizin, Hospital Essen, Germany. |
| Databáze: | OpenAIRE |
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