A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology

Autor: Shaul Druckmann, Kayvon Daie, Bei Jung Lin, Ziqiang Wei, Karel Svoboda, Tsai Wen Chen
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Fluorescence-lifetime imaging microscopy
QH301-705.5
Population
Models
Neurological
Action Potentials
Biology
Imaging data
Cellular and Molecular Neuroscience
Mice
03 medical and health sciences
Neural activity
0302 clinical medicine
Calcium imaging
Neuroimaging
Genetics
Animals
Fluorescent protein
Biology (General)
education
Molecular Biology
Neuronal population
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Neurons
education.field_of_study
0303 health sciences
Ecology
Optical Imaging
Dynamics (mechanics)
Electrophysiological Phenomena
Frontal Lobe
Molecular Imaging
Electrophysiology
030104 developmental biology
Computational Theory and Mathematics
Modeling and Simulation
Calcium
Spike (software development)
Neuroscience
030217 neurology & neurosurgery
Zdroj: PLoS Computational Biology, Vol 16, Iss 9, p e1008198 (2020)
ISSN: 1553-7358
Popis: Calcium imaging with fluorescent protein sensors is widely used to record activity in neuronal populations. The transform between neural activity and calcium-related fluorescence involves nonlinearities and low-pass filtering, but the effects of the transformation on analyses of neural populations are not well understood. We compared neuronal spikes and fluorescence in matched neural populations in behaving mice. We report multiple discrepancies between analyses performed on the two types of data, including changes in single-neuron selectivity and population decoding. These were only partially resolved by spike inference algorithms applied to fluorescence. To model the relation between spiking and fluorescence we simultaneously recorded spikes and fluorescence from individual neurons. Using these recordings we developed a model transforming spike trains to synthetic-imaging data. The model recapitulated the differences in analyses. Our analysis highlights challenges in relating electrophysiology and imaging data, and suggests forward modeling as an effective way to understand differences between these data.
Databáze: OpenAIRE
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