Is crab duplex-specific nuclease a member of the Serratia family of non-specific nucleases?
| Autor: | Alex S. Shcheglov, Veronika E. Anisimova, Ekaterina A. Bogdanova, Alexey N. Nekrasov, Dmitry A. Shagin, Denis V. Rebrikov, Ekaterina V. Barsova, Sergey Lukyanov |
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| Rok vydání: | 2008 |
| Předmět: |
Genetics
Nuclease Binding Sites Deoxyribonucleases Serratia Molecular Structure biology Brachyura Sequence analysis RNA General Medicine biology.organism_classification Protein Structure Tertiary chemistry.chemical_compound chemistry Phylogenetics Serratia marcescens Mutagenesis Site-Directed biology.protein Animals Binding site Phylogeny DNA |
| Zdroj: | Gene. 418:41-48 |
| ISSN: | 0378-1119 |
| Popis: | Kamchatka crab duplex-specific nuclease (Par_DSN) has been classified as a member of the family of DNA/RNA non-specific beta-beta-alpha metal finger (bba-Me-finger) nucleases, the archetype of which is the nuclease from Serratia marcescens. Although the enzyme under investigation seems to belong to the family of S. marcescens nucleases, Par_DSN exhibits a marked preference for double-stranded DNA as a substrate and this property is unusual for other members of this family. We have searched other Arthropod species and identified a number of novel Par_DSN homologs. A phylogenetic analysis demonstrates that the Par_DSN-like enzymes constitute a separate branch in the evolutionary tree of bba-Me-finger nucleases. Combining sequence analysis and site-directed mutagenesis, we found that Par_DSN and its homologs possess the nuclease domain that is slightly longer than that of classic Serratia relatives. The active site composition of Par_DSN is similar but not identical to that of classic Serratia nucleases. Based on these findings, we proposed a new classification of Par_DSN-like nucleases. |
| Databáze: | OpenAIRE |
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