Prostate-specific membrane antigen: evidence for the existence of a second related human gene
| Autor: | Kenneth A. MacLennan, B.H. Maraj, S Andersen, Nicholas Lench, J Cross, J Leek, Ian M. Carr, David Meredith, A. Bailey, P Whelan, Alex F. Markham |
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| Rok vydání: | 1995 |
| Předmět: |
Glutamate Carboxypeptidase II
Male Cancer Research Genetic enhancement Molecular Sequence Data 030232 urology & nephrology Biology Polymerase Chain Reaction 03 medical and health sciences Prostate cancer 0302 clinical medicine Antigens Neoplasm Prostate Yeasts LNCaP medicine Glutamate carboxypeptidase II Humans Cloning Molecular Gene In Situ Hybridization Fluorescence Base Sequence Chromosomes Human Pair 11 fungi Chromosome Mapping Prostatic Neoplasms DNA Neoplasm medicine.disease Molecular biology 3. Good health Blotting Southern Prostate-specific antigen medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Antigens Surface Human genome Chromosomes Fungal Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1995.377 |
| Popis: | Prostate-specific membrane antigen (PSM) is a glycoprotein recognised by the prostate-specific monoclonal antibody 7E11-C5, which was raised against the human prostatic carcinoma cell line LNCaP. A cDNA clone for PSM has been described. PSM is of clinical importance for a number of reasons. Radiolabelled antibody is being evaluated both as an imaging agent and as an immunotherapeutic in prostate cancer. Use of the PSM promoter has been advocated for gene therapy applications to drive prostate-specific gene expression. Although PSM is expressed in normal prostate as well as in primary and secondary prostatic carcinoma, different splice variants in malignant tissue afford the prospect of developing reverse transcription-polymerase chain reaction (RT-PCR)-based diagnostic screens for the presence of prostatic carcinoma cells in the circulation. We have undertaken characterisation of the gene for PSM in view of the protein's interesting characteristics. Unexpectedly, we have found that there are other sequences apparently related to PSM in the human genome and that PSM genomic clones map to two separate and distinct loci on human chromosome 11. Investigation of the function of putative PSM-related genes will be necessary to enable us to define fully the role of PSM itself in the development of prostatic carcinoma and in the clinical management of this malignancy. Images Figure 1 Figure 3 Figure 4 Figure 5 Figure 6 |
| Databáze: | OpenAIRE |
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