Light signals counteract alterations caused by simulated microgravity in proliferating plant cells

Autor: Raúl Herranz, F. Javier Medina, Veronica Pereda-Loth, Aránzazu Manzano, Julio Sáez-Vásquez, Anne de Bures
Přispěvatelé: Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Groupement scientifique de Biologie et de Medecine Spatiale (GSBMS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National d'Études Spatiales [Toulouse] (CNES), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), Agencia Estatal de Investigación (España), European Commission, Manzano, Aranzazu, Pereda-Loth, Veronica, Sáez-Vásquez, J., Herranz, Raúl, Medina, F. Javier, Manzano, Aranzazu [0000-0002-0150-0803], Pereda-Loth, Veronica [0000-0002-7365-6217], Sáez-Vásquez, J. [0000-0002-2717-7995], Herranz, Raúl [0000-0002-0246-9449], Medina, F. Javier [0000-0002-0866-7710]
Rok vydání: 2021
Předmět:
0106 biological sciences
abiotic stress
Meristem
Arabidopsis
ribosome biogenesis
Plant Science
Cell cycle
space plant biology
Biology
Plant Roots
01 natural sciences
03 medical and health sciences
nucleolin
Auxin
Plant Cells
Genetics
Arabidopsis thaliana
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Nucleolin
chemistry.chemical_classification
photoperiodism
0303 health sciences
Space plant biology
Weightlessness
Cell growth
fungi
auxin transport
Auxin transport
food and beverages
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
Space Flight
15. Life on land
Abiotic stress
Plant cell
biology.organism_classification
Cell biology
chemistry
Seedlings
Ribosome biogenesis
root meristem
Darkness
cell cycle
Graviresponse
Root meristem
graviresponse
010606 plant biology & botany
Zdroj: American Journal of Botany
American Journal of Botany, Botanical Society of America, In press, ⟨10.1002/ajb2.1728⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1537-2197
0002-9122
Popis: 18 p.-9 fig.
Premise: Light and gravity are fundamental cues for plant development. Our understanding of the effects of light stimuli on plants in space, without gravity, is key to providing conditions for plants to acclimate to the environment. Here we tested the hypothesis that the alterations caused by the absence of gravity in root meristematic cells can be counteracted by light.
Methods: Seedlings of wild‐type Arabidopsis thaliana and two mutants of the essential nucleolar protein nucleolin (nuc1, nuc2) were grown in simulated microgravity,either under a white light photoperiod or under continuous darkness. Key variables of cell proliferation (cell cycle regulation), cell growth (ribosome biogenesis),and auxin transport were measured in the root meristem using in situ cellular markers and transcriptomic methods and compared with those of a 1 g control.
Results: The incorporation of a photoperiod regime was sufficient to attenuate or suppress the effects caused by gravitational stress at the cellular level in the root meristem. In all cases, values for variables recorded from samples receiving light stimuli in simulated microgravity were closer to values from the controls than values from samples grown in darkness. Differential sensitivities were obtained for the two nucleolin mutants.
Conclusions: Light signals may totally or partially replace gravity signals, significantly improving plant growth and development in microgravity. Despite that, molecular alterations are still compatible with the expected acclimation mechanisms, which need to be better understood. The differential sensitivity of nuc1 and nuc2 mutants to gravitational stress points to new strategies to produce more resilient plants to travel with humans in new extraterrestrial endeavors.
This work was funded by the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation, Grants#ESP2015‐64323‐R and #RTI2018‐099309‐B‐I00 (co‐funded by EU‐ERDF) to F.J.M., and Bonus Recherche from the UPVD to J.S.V. The use of the facilities of microgravity simulation was provided by the ESA‐CORA‐Ground Based Facilities Program, contract Ref. #4000105761 to F.J.M. and R.H. A.M. was recipient of a contract of the Program for Young Researchers Training of the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation Ref. #BES‐2013‐063933.
Databáze: OpenAIRE
Externí odkaz: