Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
Autor: | Raúl Herranz, F. Javier Medina, Veronica Pereda-Loth, Aránzazu Manzano, Julio Sáez-Vásquez, Anne de Bures |
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Přispěvatelé: | Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Groupement scientifique de Biologie et de Medecine Spatiale (GSBMS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National d'Études Spatiales [Toulouse] (CNES), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), Agencia Estatal de Investigación (España), European Commission, Manzano, Aranzazu, Pereda-Loth, Veronica, Sáez-Vásquez, J., Herranz, Raúl, Medina, F. Javier, Manzano, Aranzazu [0000-0002-0150-0803], Pereda-Loth, Veronica [0000-0002-7365-6217], Sáez-Vásquez, J. [0000-0002-2717-7995], Herranz, Raúl [0000-0002-0246-9449], Medina, F. Javier [0000-0002-0866-7710] |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
abiotic stress Meristem Arabidopsis ribosome biogenesis Plant Science Cell cycle space plant biology Biology Plant Roots 01 natural sciences 03 medical and health sciences nucleolin Auxin Plant Cells Genetics Arabidopsis thaliana Ecology Evolution Behavior and Systematics 030304 developmental biology Nucleolin chemistry.chemical_classification photoperiodism 0303 health sciences Space plant biology Weightlessness Cell growth fungi auxin transport Auxin transport food and beverages [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics Space Flight 15. Life on land Abiotic stress Plant cell biology.organism_classification Cell biology chemistry Seedlings Ribosome biogenesis root meristem Darkness cell cycle Graviresponse Root meristem graviresponse 010606 plant biology & botany |
Zdroj: | American Journal of Botany American Journal of Botany, Botanical Society of America, In press, ⟨10.1002/ajb2.1728⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 1537-2197 0002-9122 |
Popis: | 18 p.-9 fig. Premise: Light and gravity are fundamental cues for plant development. Our understanding of the effects of light stimuli on plants in space, without gravity, is key to providing conditions for plants to acclimate to the environment. Here we tested the hypothesis that the alterations caused by the absence of gravity in root meristematic cells can be counteracted by light. Methods: Seedlings of wild‐type Arabidopsis thaliana and two mutants of the essential nucleolar protein nucleolin (nuc1, nuc2) were grown in simulated microgravity,either under a white light photoperiod or under continuous darkness. Key variables of cell proliferation (cell cycle regulation), cell growth (ribosome biogenesis),and auxin transport were measured in the root meristem using in situ cellular markers and transcriptomic methods and compared with those of a 1 g control. Results: The incorporation of a photoperiod regime was sufficient to attenuate or suppress the effects caused by gravitational stress at the cellular level in the root meristem. In all cases, values for variables recorded from samples receiving light stimuli in simulated microgravity were closer to values from the controls than values from samples grown in darkness. Differential sensitivities were obtained for the two nucleolin mutants. Conclusions: Light signals may totally or partially replace gravity signals, significantly improving plant growth and development in microgravity. Despite that, molecular alterations are still compatible with the expected acclimation mechanisms, which need to be better understood. The differential sensitivity of nuc1 and nuc2 mutants to gravitational stress points to new strategies to produce more resilient plants to travel with humans in new extraterrestrial endeavors. This work was funded by the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation, Grants#ESP2015‐64323‐R and #RTI2018‐099309‐B‐I00 (co‐funded by EU‐ERDF) to F.J.M., and Bonus Recherche from the UPVD to J.S.V. The use of the facilities of microgravity simulation was provided by the ESA‐CORA‐Ground Based Facilities Program, contract Ref. #4000105761 to F.J.M. and R.H. A.M. was recipient of a contract of the Program for Young Researchers Training of the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation Ref. #BES‐2013‐063933. |
Databáze: | OpenAIRE |
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