Separation of viable histamine-producing bacteria from yellowtail meat components by density gradient centrifugation
| Autor: | Emiko Araki, Hideaki Matsuoka, Kodai Takayama, Mikako Saito |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Differential centrifugation
Morganella morganii Chromatography biology Density gradient Public Health Environmental and Occupational Health Ficoll biology.organism_classification Applied Microbiology and Biotechnology Microbiology Perciformes chemistry.chemical_compound chemistry Fish Products Centrifugation Density Gradient Food Microbiology Food microbiology Animals Centrifugation Histamine Bacteria Food Analysis |
| Zdroj: | Biocontrol science. 14(1) |
| ISSN: | 1342-4815 |
| Popis: | Quantitative separation of live cells from food samples is essential for non-culture methods to be validated. In this viewpoint, the feasibility of density gradient centrifugation (DGC) was demonstrated for the first time using samples of yellowtail meat to which Morganella morganii, a histamine producing bacterium had been added. Using a Ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of M. morganii cells were collected in 20-50 w/v % layers. The total cell collection rate ranged from 73-86 % irrespective of the cell density in the range 10(2)-10(6) cells/200 microl. |
| Databáze: | OpenAIRE |
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